摘要
目的 :观察组成“膝腱 脊髓 膀胱”反射弧的神经细胞 ,从形态学上寻找反射弧重建膀胱功能的证据。方法 :5只犬复制成L5 S2前根交叉吻合动物模型后 ,辣根过氧化物酶逆向追踪显示膀胱新支配神经的脊髓中枢 ,并进行吻合口再生神经纤维数量的计算机图像分析。结果 :辣根过氧化物酶标记阳性细胞出现在L5前角 ,以α运动神经元为主 ;吻合口可见较多的神经纤维通过。结论 :形态学上证实“膝腱 脊髓 膀胱”反射弧可以通过L5 S2前根交叉吻合得以建立 。
Objective:To observe the nerve cell composed of 'knee tendon spinal cord bladder' reflex arc and it′s morphological character,so as to search the morphological evidence of the bladder reconstruction with the reflex arc.Method:As the animal model have been established in 5 dogs by L5 S2 ventral roots crossover anastomosis,neural tracing study with horseradish peroxidase (HRP) were preceded and the morphology of the positive cells together with regeneration axons were analyzed with computer picture analysis system.Result:The new regenerating axons can pass through anastomosis areas and their number meets the requirement of bladder functional reconstruction,as well as,HRP positive cells appeared in L5 ventral horn and like somatic motor nerve cell morphologically.Conclusion:It was morphologically confirmed that 'knee tendon spinal cord bladder' reflex arc and bladder functional reconstruction can be established by L5 S2 anterior roots anastomosis and the spinal cord in the reflex arc is composed of a group of somatic motor cells located in L5 ventral horn.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2002年第4期273-276,共4页
Chinese Journal of Spine and Spinal Cord
关键词
脊髓损伤
膀胱功能重建
神经再支配
形态学
反射弧
犬
Spinal cord injury(SCI)
Bladder functional reconstruction
Reinnervation
Morphology
Reflex arc
Dog
