摘要
目的观察不同功率高强度聚焦超声(HIFU)治疗人胰腺癌移植瘤的有效性和安全性。方法建立裸小鼠人胰腺癌YY-1细胞移植瘤模型,将荷瘤鼠分为低功率HIFU治疗组(200 W,n=10)、高功率HIFU治疗组(300 W,n=10)和空白对照组(n=10)。观察肿瘤体积变化、肿瘤生长速率及治疗不良反应,末端脱氧核苷酸转移酶、生物素d UTP切口末端标记(TUNEL)法检测各组肿瘤细胞凋亡率。结果低功率组、高功率组肿瘤体积、生长速率均明显低于对照组(P<0.05),两治疗组间比较差异无统计学意义(P>0.05)。与低功率组相比,高功率组不良反应增多(P<0.05),主要为皮肤灼伤(60%)和声通道损伤(20%)。治疗后7 d、14 d,低功率组和高功率组细胞凋亡率均高于对照组(P<0.05),两治疗组间细胞凋亡率差异无统计学意义(P>0.05)。结论低功率HIFU治疗胰腺癌移植瘤有效,且安全性更好。
Objective To investigate the efficacy and safety of different acoustic power of high-intensity focused ultrasound（HIFU） in treating human pancreatic xenograft models. Methods Human pancreatic cancer cells（YY- 1） were implanted subcutaneously in nude mice to establish animal models. The tumor bearing mice were divided into low-power HIFU treatment group（200 W,n =10）, high- power HIFU treatment group（300 W,n=10） and blank control group（n=10）. The change of tumor volume, the tumor growth rate and side effects were recorded. The apoptosis rate of tumor cells of each group was determined by TUNEL method. Results The tumor volume and the tumor growth rate of the low- power group and the high- power group were significantly lower than those of the control group（P〈 0.05）, while no statistically significant differences in the tumor volume and the tumor growth rate existed between the low-power group and the high-power group（P〉0.05）. Compared with the low-power group, the incidence of side effects in the highpower group was significantly higher（P〈0.05）, including mainly skin burn（60%） and acoustic channel injury（20%）. At the 7th and 14 th day after the treatment, the apoptosis rates of tumor cells in both the low-power group and the high-power group were significantly higher than that of the control group（P0.05）, but the difference in the apoptosis rates of tumor cells was not statistically significant between the low- power group and the high-power group（P〉0.05）. Conclusion For the treatment of human pancreatic xenograft tumor in nude mice models, HIFU with low power is effective and safer.
出处
《介入放射学杂志》
CSCD
北大核心
2015年第7期612-615,共4页
Journal of Interventional Radiology
基金
天津市滨海新区卫生局重点支持项目(2011BHKZ009)
关键词
高强度聚焦超声
胰腺癌
凋亡
high-intensity focused ultrasound
pancreatic cancer
apoptosis
