摘要
水通道蛋白(AQPs)是细胞间和细胞内水分运输的主要通道,对于植物细胞的水分稳态和胁迫响应具有重要作用。本研究从香蕉中克隆了一个水通道蛋白基因Ma PIP2-2。序列分析结果表明,该基因的开放阅读框(ORF)为846 bp,编码281个氨基酸。多序列比对和进化树分析结果表明,该基因编码的蛋白与其它植物中水通道蛋白具有较高的一致性,并且与拟南芥At PIP2-2和At PIP2-3的亲缘关系最近。亚细胞定位表明该基因定位在细胞膜上。实时荧光定量PCR分析结果表明,该基因能够在转录水平响应甘露醇、低温、Na Cl胁迫和ABA信号。这些结果表明Ma PIP2-2可能参与了非生物逆境胁迫应答,为进一步研究Ma PIP2-2基因的功能鉴定了基础。
Water channel protein, （Aquaporins, AQPs）, which was the main water transport corridor, plays a major role in maintaining water steady state and the stress response. In the present study, a A QP gene designated MaPIP2-2 from banana was isolated. MaPIP2-20RF was 846 bp, encoding 281 amino acids. Comparison of amino acid sequences and phylogenetie analysis indicated that MaPIP2-2 was closely related with AtPIP2-2 and AtPIP2- 3. Subeellular localization assays showed that the MaPIP2-2 protein was present in the plasma membrane. Real- time quantitative polymerase chain reaction （QPCR） assay revealed that MaPIP2-2 participated in the response of abiotic stresses at transcriptional level. These results indicated that MaPIP2-2 might be involved in abiotie stress responses. The study would lay a foundation for further investigating the function of MaPIP2-2.
出处
《热带作物学报》
CSCD
北大核心
2015年第7期1267-1273,共7页
Chinese Journal of Tropical Crops
基金
现代农业产业技术体系(国家香蕉产业技术体系)建设专项资金资助(No.CARS-32)
973计划前期研究专项(No.2014CB160314)
