摘要
探讨了溶血磷脂酰乙醇胺(LPE)硅胶柱色谱纯化的可行性。通过选择,确定以硅胶为固定相、氯仿-甲醇(体积比2∶1)为洗脱液,进行LPE纯化工艺优化。得到的最优工艺条件为:上样量1∶40(样品质量与固定相质量比),上样液质量浓度30 mg/m L,洗脱流速2.5 m L/min;在最佳工艺条件下,最终LPE产品纯度为99.05%,回收率为88.69%。研究为进一步探讨工业化制备LPE提供了基础数据和理论支撑。
The feasibility of purification of lysophosphatidylethanolamine （LPE） using silica gel column chromatography was studied. Through selection, choosing silica gel as stationary phase and chloroform - methanol（volume ratio 2：1 ）as eluent, the purification process of LPE was optimized as follows： sample amount 1：40 （mass ratio of sample to stationary phase）, mass concentration of sample 30 mg/mL, flow rate of eluent 2.5 mL/min; under these conditions, the purity and recovery rate of LPE were 99.05% and 88.69%, respectively. The study provided fundamental data and theoretical support for the further industrial preparation of LPE.
出处
《中国油脂》
CAS
CSCD
北大核心
2015年第7期64-68,共5页
China Oils and Fats
基金
国家自然科学基金(31301502)
河南省农业科学院优秀青年科技基金(2013YQ25)
关键词
磷脂酰乙醇胺
溶血磷脂酰乙醇胺
硅胶柱色谱
纯化
phosphatidylethanolamine
lysophosphatidylethanolamine
silica gel column chromatography
purification
