摘要
目的是采用HPLC法建立萹蓄配方颗粒的指纹图谱分析方法。采用HPLC法,以Megres5-C18（Hanbon,250 mm×4.6 mm,5μm）为色谱柱,以乙腈-0.1%磷酸溶液为流动相梯度洗脱;检测波长为0~15 min,260 nm,15~65 min,350 nm;流速为1.0 m L/min,柱温为30℃。结果表明,构建的指纹图谱共有7个共有峰,各批次萹蓄配方颗粒样品指纹图谱与对照指纹图谱相似度均在0.95以上,可用于萹蓄配方颗粒的定性鉴别。结论是,建立的指纹图谱重现性好,可为提高萹蓄配方颗粒的质量控制提供有益的信息。
The purpose is to use fingerprint analysis method Polygonum aviculare granules formulation H_PLC method. HPLC method to Megres5-C18 （Hanbon, 250 mm × 4.6 mm, 5 lana ） was with acetonitrile -0.1% phosphoric acid mobile phase gradient elution; detection wavelength was 0 × 15 min, 260 nm, 15 × 65 min, 350 rim; flow rate of 1.0 mL / rain, the column temperature was 30 ℃. The results showed that the establishment of Fingerprint 7 common peaks, each batch Polygonum aviculare granule formulation and the control sample fingerprint similarity was above 0.95 can be used to build Formula Granules Polygonum qualitative identification. It concluded that the establishment of fingerprint reproducible can improve the quality of Polygonum aviculare Formula Granules provide useful information control. Key words： Polygonum aviculare formula particles; I-IPLC method; fingerprints; peaks
出处
《科技与创新》
2015年第15期13-14,共2页
Science and Technology & Innovation
基金
2011年度江苏省企业博士聚集计划资助项目
关键词
萹蓄配方颗粒
HPLC法
指纹图谱
特征峰
Polygonum aviculare formula particles
HPLC method
fingerprints
peaks
