摘要
为了探讨中国肾茶种质资源的遗传多样性,本研究采用100条ISSR引物对87份肾茶种质资源进行试验分析,发现20条引物能扩增出清晰条带.在87份供试材料中,20个ISSR引物共产生144条扩增条带,其中多态性条带120条,多态率83.3％,平均每个ISSR标记能产生6.0条多态性片段.87份材料间的遗传相似系数变化范围为0.48 ～0.98,但6份栽培品种间遗传相似系数为0.88 ～ 0.98显示出栽培种的遗传基础相对狭窄.利用UPGMA聚类分析,供试材料可聚为2类,其中栽培品种单独聚成1类,野生材料聚为1类.结果显示,中国野生肾茶种质资源遗传多样性丰富,具有较高的开发利用价值.
In order to analyze the genetic diversity of Clerodendranthus spicatus （ Thunb. ） C. Y. Wu. in China, a total of 87 accessions of C. spicatus （Thunb.） C. Y. Wu were analyzed by using ISSR markers. One hundred of ISSR primers were designed to produce amplification products of the accessions, among which 20 primers pro- duced distinct amplification products. The 20 primers yielded a total of 144 bands in the 87 accessions, of which 120 bands （83.3 % ） were polymorphic （6.0 polymorphic bands per primer）. The ISSR-based genetic similarity （GS） values of the 87 C. spicatus accessions ranged from 0.48 to 0.98, but the GS values of six accessions of cultivars ranged from 0.88 - 0.98. This result indicated that the genetic basis was comparatively narrow among the cultivars. Moreover, the 87 accessions were clustered into two groups by UPGMA, among which the cuhivars were classified into one group and the wild species into another group. It is concluded that the wild germplasm resources of C. spicatus in China are more genetically diverse and hence have a great potential for further exploi- tation and utilization.
出处
《热带生物学报》
2015年第2期204-222,共19页
Journal of Tropical Biology
基金
海南省星火产业带专项"海南肾茶高效栽培与示范"(HNXH201319)
海南省产学研一体化专项"海南木棉产业发展关键技术集成与示范"(CXY20140027)
国家级大学生创新创业训练计划项目"爱吾园生物除醛创艺坊"(201421)
海南省中西部高校提升综合实力工作资金项目"农艺应用型本科实践教学创新团队"(201417)
海南大学应用科技学院(儋州校区)基金项目"裂掌榕种质资源调查及资源圃的建立"(HYK1524)
关键词
肾茶
CTAB法
ISSR
遗传多样性
Clerodendranthus spicatus （Thunb.） C. Y. Wu.
CTAB methods
ISSR
genetic diversity
