摘要
为深入探究‘大白谷’(Oryza sativa L.ssp.Indica)感病的分子机理,以及抗病基因在发病过程中所起的作用,克隆了‘大白谷’CC-NBS-LRR蛋白编码基因Os11g RGA8,对Os11g RGA8基因进行了生物信息学分析,并对其在水稻抗干尖线虫侵染过程中的功能进行了研究。Os11g RGA8基因位于11号染色体,其编码蛋白含有733个氨基酸,等电点为5.99,相对分子质量为84355.07。序列分析表明该氨基酸序列含有RX-CC、AAA_22、NBS和LRR_4这4个结构域,Os11g RGA8基因编码抗病蛋白属于CC-NBSLRR抗病蛋白家族。Q-PCR结果显示,接种水稻干尖线虫SAMN02420038的籼型常规稻‘大白谷’,与CK组‘大白谷’相比,Os11g RGA8基因表达量表现为上调,且24 h内上调增幅较缓,48 h时上调增幅突然上涨,72 h时上调增幅又出现下降,表明该基因参与‘大白谷’天然免疫反应过程,在水稻干尖线虫侵染过程中起到一定抗性作用。
Os11g RGA8 gene was cloned and its bioinformatics were analyzed in order to explore the molecularmechanism of the indica rice（Oryza sativa L. ssp. Indica） and the role of resistance genes in the course ofillness. Bioinformatics of Os11 g RGA8 gene was analyzed, and its function in the process of Aphelenchoides besseyi infection was studied. Os11 g RGA8 genes were in chromosome 11, containing 733 amino acids, itscoding protein isoelectric point was 5.99 and the relative molecular mass was 84355.07. The sequence analysisshowed that four structure domains RX- CC, AAA_22, NBS and LRR_4 were contained in its amino acidsequence and the disease-resistant protein encoded by Os11 g RGA8 gene belonged to CC-NBS-LRR disease-resistant protein family. Q- PCR results showed that, compared with CK rice seedlings group, the Indicainoculated by A. besseyi SAMN02420038 got a raised quantity of Os11 g RGA8 gene expression and within 24 hthe increase was relatively slow. Then the growth rose suddenly in 48 h, but the increase declined in 72 h,indicating that Os11 g RGA8 gene was involved in Indica natural immune reaction process and played a role inthe process of A. besseyi resistance infection.
出处
《中国农学通报》
2015年第21期279-283,共5页
Chinese Agricultural Science Bulletin
基金
高等学校博士学科点专项科研基金资助课题(博士点新教师基金资助项目)"水稻干尖线虫诱导水稻防卫反应基因分析"(20100062120002)
大学生创新性实验计划项目"植物抗病基因研究"(201410225162)
