摘要
目的:通过Box-Behnken效应面法优化川芎的提取工艺。方法:HPLC测定川芎饮片和药液中川芎嗪、阿魏酸、藁本内酯的含量,以川芎嗪、阿魏酸、藁本内酯转移率的多指标综合加权评分(以层次分析法确定其权重系数为0.6∶0.3∶0.1)为指标,通过Box-Behnken试验考察溶剂倍数、提取时间、乙醇浓度等因素的影响,对结果进行二项式方程拟合,利用效应面法优化提取工艺并进行预测分析。结果:川芎的最佳提取工艺为饮片浸泡1 h,以4倍量50%乙醇回流提取2次,每次2 h,预测值与验证值偏差较小。结论:该工艺稳定可行,实际值与预测值吻合度高,预测性良好,优选的工艺各成分提取率高,为川芎及其制剂的研究提供了实验依据。
Objective ： To optimize extraction process of Ligusticum chuanxiong Hort by analytic hierarchy process and Box - Behnken design - response surface method. Method ： The contents of ligustrazine, ferulic acid and ligustilide were de- termined by HPLC. Independent variable included concentraction of ethanol, liquid - solid ratio and extraction time and dependent variable included the muhi - index comprehensive weighted scoring method of extraction rate of ligustrazine, ferulic acid and ligustilide （ With analytic hierarchy process to determine the weight coefficient is 0.6 ： 0.3 ： 0.1 ）. No - linear mathematic models were to estimate the relationship between independent and dependent variables and respone sur- face methodology was used to optimize extraction process and compare the observed and predicted values as prediction.; Results：Optimum estraction technology was listed as follows： Yinpian soak 1 h,4 times more than the volumn of 50% eth- anol solution,by heating reflux extraction for twice, each time 2 h. Deviation between the observed and predicted values was small. Conclusion：The optimized extraction technology was stable and feasible with high predictability, and it provides an experimental basis for compound preparation technology research of Ligusticum chuanxiong Hort.
出处
《中华中医药学刊》
CAS
北大核心
2015年第7期1706-1709,I0014,共5页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省科技厅公益性技术应用研究计划项目(2014C33216)
关键词
川芎
提取工艺
层次分析
BOX-BEHNKEN效应面法
Ligusticum chuanxiong Hort
extraction technology
analytic hierarchy process
Box - Behnken design -response surface methodology
