摘要
目的研究芳香化浊液的质量标准,并建立质量控制的方法。方法处方中广藿香和紫苏叶采用薄层色谱法进行定性鉴别;制剂中的百秋李醇和薄荷脑采用气相色谱法同时进行含量测定的研究,毛细管柱,FID检测器,以氮气为载气,流速：1.5 ml·min-1,柱温：初始温度110℃,以5℃·min-1的升温速率到170℃,再以50℃·min-1的升温速率到220℃,保持3 min;进样口温度：200℃,检测器温度：250℃,分流比：20：1,恒流模式。结果广藿香和紫苏叶的薄层色谱斑点明显清晰,阴性对照没有干扰;百秋李醇进样量在0.0125 6-0.200 9μg（r=0.999 8）呈良好线性关系;平均回收率为99.18%,RSD=1.07%;薄荷脑进样量在0.024 97-0.399 5μg（r=0.999 2）呈良好线性关系;平均回收率为99.12%,RSD=1.13%。结论方法学研究结果表明芳香化浊液的质量控制方法精确,专属性强,重现性好。
Objective To research the quality standard of Fangxiang Huazhuo Ye and establish the qualitative and quantitative method. Methods Thin-layer chromatography was used for identifying the Herba Pogostemonis and Folium Perillae; GC method was used for quantitative analysis of patchouli alcohol and menthol. Capillary column, FID detector, with nitrogen as the carrier,Velocity：1.5 ml·min-1,column temperature：initial temperature 110 ℃,with 5℃·min-1 heating rate to 170 ℃, and 50 ℃·min-1 to 220 ℃, keep 3 min, injection port temperature： 200 ℃,detector temperature： 250 ℃, split ratio ： 20 ： 1, constant current mode. Results The spots of the Herba Pogostemonis and Folium Perillae were clear, negative control without interference by TLC; A good linearity of patchouli alcohol was in range of 0.012 56 - 0.200 9 μg（r = 0.999 8）with the peak area. The average recovery of patchouli alcohol was 99.18% with RSD = 1.07%. A good linearity of menthol was in range of 0.024 97 -0.399 5 μg（r = 0.999 2）with the peak area. The average recovery of menthol was 99.12% with RSD = 1.13%.Conclusion The methodology of the research results show that the quality control method of Fangxiang Huazhuo Ye was accurate,exclusive, and with good reproducibility.
出处
《现代中药研究与实践》
CAS
2015年第3期63-65,74,共4页
Research and Practice on Chinese Medicines
基金
东莞市科技计划医疗卫生类科研项目(2010105150032)
