摘要
目的：探讨急性胰腺炎患者外周血中性粒细胞明胶酶相关脂质运载蛋白（NGAL）和内源性脑肠肽（Ghrelin）水平变化及其意义。方法将104例急性胰腺炎患者根据病情分为轻型胰腺炎组（n ＝65）和重型胰腺炎组（n ＝39），收集所有研究对象临床资料，利用酶联免疫吸附试验（ELISA 法）对血浆中 NGAL 和Ghrelin 水平进行检测。结果重型胰腺炎组血浆 NGAL 和 Ghrelin 水平分别为（15．05±5．32）nmol／L 和（349．7±68．5）ng／L，均高于轻型胰腺炎组，差异均有统计学意义（t ＝10．239、14．453，均 P ＜0.05）；相关分析显示，急性胰腺炎患者血浆 NGAL 水平与白细胞、C 反应蛋白、APACHEⅡ评分、BISAP 指数、CTSI 指数和住院时间均呈正相关（r ＝0．447、0．335、0．626、0．534、0．497和0．372，均 P ＜0．05），Ghrelin 水平与白细胞、C 反应蛋白、APACHEⅡ评分、BISAP 指数和 CTSI 指数均呈正相关（r ＝0．392、0．407、0．592、0．481和0．506，均 P ＜0.05）；ROC 曲线显示，血浆 NGAL 水平在诊断重型胰腺炎时的灵敏度79．5％，特异度93.8％，曲线下面积0.899（95％CI：0．828-0．970），血浆 Ghrelin 分别为89．7％、90．8％和0.946（95％CI：0.897-0．995）。结论NGAL 和 Ghrelin 水平在重型胰腺炎患者中显著升高，且与病情严重程度呈正相关，在早期诊断重型胰腺炎时具有较好的诊断效能。
Objective To investigate the changes of neutrophil gelatinase -associated lipocalin （NGAL） and Ghrelin levels in peripheral blood of patients with acute pancreatitis and their significance.Methods 104 cases of acute pancreatitis were divided into mild acute pancreatitis group （n =65 ）and severe acute pancreatitis group （n =39）,according to the conditions of the patients.The clinical indexes of all subjects were collected.The levels of NGAL and Ghrelin in plasma were detected by enzyme -linked immunosorbent assay （ELISA method）.Results The levels of NGAL and Ghrelin in severe acute pancreatitis group were （15.05 ±5.32）nmol/L and （349.7 ± 68.5）ng/L,respectively,which were higher than mild acute pancreatitis group,the differences were statistically signif-icant （t =10.239 and 14.453,P 〈0.05）.Correlation analysis showed that the levels of NGAL in acute pancreatitis were positively correlated with white blood cell levels,C -reactive protein levels,APACHEⅡ score,BISAP index,CT-SI index and length of stay （r =0.447,0.335,0.626,0.534,0.497 and 0.372,all P 〈0.05）,and the levels of Gh-relin in acute pancreatitis were positively correlated with white blood cell levels,C -reactive protein levels,APACHEⅡ score,BISAP index and CTSI index （r =0.392,0.407,0.592,0.481 and 0.506,all P 〈0.05 ）.ROC curve showed that the levels of NGAL levels in the diagnosis of severe pancreatitis,the sensitivity was 79.5%,specificity was 93.8%,and area under the curve was 0.899 （95% CI：0.828 -0.970）,plasma Ghrelin were 89.7%,90.8%, and 0.946 （95 % CI：0.897 -0.995）,respectively.Conclusion The levels of NGAL and Ghrelin are significantly elevated in patients with severe acute pancreatitis,and they are positively correlated with the severity of patients＆#39;con-ditions.They have good diagnostic performance in early diagnosis of severe pancreatitis.
出处
《中国基层医药》
CAS
2015年第13期2019-2022,共4页
Chinese Journal of Primary Medicine and Pharmacy
