摘要
目的探讨黄芪甲苷对肺纤维化(PF)小鼠肺组织氧化应激损伤的保护作用。方法 120只C57BL/6J小鼠采用气管内穿刺注入博莱霉素(BLM)法建立PF小鼠模型,随机分为假手术组、模型组、N-乙酰半胱氨酸组[250 mg/(kg·d)],以及黄芪甲苷高[100 mg/(kg·d)]、中[50 mg/(kg·d)]、低[25 mg/(kg·d)]剂量组,每组20只,分别给予相应药物灌胃,假手术组与模型组予等量生理盐水灌胃,连续21天。于造模后第3、7、14和21天处死动物,观察各组肺组织病理学变化,检测肺组织羟脯氨酸(HYP)、谷胱甘肽(GSH)、丙二醛(MDA)含量及支气管肺泡灌洗液(BALF)中转化生长因子(TGF-β1)的水平。结果与假手术组比较,模型组小鼠术后不同时间点体重明显下降,肺泡炎分级和肺纤维化程度均明显增加,肺组织HYP、MDA含量明显增加(P<0.05),GSH含量明显降低(P<0.05),且随术后时间延长不断增加,而BALF中TGF-β1含量也明显高于假手术组(P<0.05)。与模型组比较,黄芪甲苷各组与N-乙酰半胱氨酸组的肺泡炎分级和肺纤维化程度均明显减轻,肺组织HYP、MDA含量和BALF中TGF-β1均降低,GSH含量明显增加(P<0.05)。黄芪甲苷高剂量组上述各指标与N-乙酰半胱氨酸组比较差异均无统计学意义(P>0.05)。结论高剂量黄芪甲苷能明显抑制BLM诱导的小鼠氧化应激状态,延缓PF进程。
Objective To explore the protective action of Astragaloside on oxidative stress of lung tissue in bleomycin-induced pulmonary fibrosis （PF） in mice. Methods 120 C57BL/6J mice, which were made into PF mice model by injecting BLM with endotracheal puncture, were randomly divided into a sham-operation group, a model group, a positive drug group [ N-acetyl cysteine, 250 mg/（kg · d）], and Astragaloside high dosage [ 100 mg/（ kg · d）], moderate dosage [50 mg/（ kg · d）], low dosage group [25 mg/（kg · d）1 of 20 in each group. Each group was given corresponding medicines with intragastric administration, the sham- operation group and the model group were given saline with intragastric administration. The treatment in all groups last for 21 days continuously. At the time points of the 3rd, 7th, 14th, 21st day after the mice model were made, the mice were killed for the observation of the pathological changes in the lung tissue and the detection of the contents of hydroxyproline （ HYP）, GSH, and MDA in the lung tissue and the level of TGF-β1 in bronchial alveolar lavage fluid （BALF）. Results Compared with the sham-operation group, the model group after operation at different time points showed the body weight decreased remarkably, alveolar inflammation grading and the degree of pulmonary fibrosis increased significantly, the content of HYP and MDA in lung tissue increased （P 〈 0. 05）, the content of GSH significantly more and more reduced （P 〈 0. 05） with the time prolonged, but TGF-β1 in BALF was higher than that in the sham-operation group （ P 〈 0. 05 ）. Compared with the model group, the Astragaloside group with different dosages and the positive drug group showed milder changes in alveolar inflammation grading and the degree of pulmonary fibrosis, and the content of HYP and MDA in lung tissue and the content of TGF-β1 in BALF reduced, the content of GSH in lung tissue increased （P 〈 0. 05 ）. The difference in the above data between the high dosage of Astragaloside group and the positive drug group had no statistic significance（ P 〉 0.05 ）. Conclusion The high dosage of Astragaloside can remarkably inhibit the status of oxidative stress induced by BLM in mice and slow down PF progress.
出处
《北京中医药》
2015年第6期484-488,共5页
Beijing Journal of Traditional Chinese Medicine
基金
北京市卫生系统高层次卫生人才培养计划(2011-3-081)
关键词
黄芪甲苷
博莱霉素
肺纤维化
病理形态
氧化应激
小鼠
Astragaloside
bleomycin
pulmonary fibrosis
pathomorphology
oxidative stress
mice
