摘要
目的：采用高效液相色谱法测定商品苦荞茶中槲皮素和山柰酚的含量．方法：回流提取法，色谱柱为DIKM A diamonsil（4.6 mm ×250 mm ，5μm），检测波长260 nm ，柱温25℃，流动相为乙腈0.1％磷酸水，梯度洗脱，流速为1 mL /min ．结果：不同类型苦荞茶中黑苦荞全胚茶的槲皮素和山柰酚含量最低（0.704～1.089 mg/g），不同生产地苦荞茶中槲皮素和山柰酚含量差异较大．结论：本试验可为苦荞茶的研究和质量标准建立提供科学依据．
In order to provide a scientific basis for the establishment of quality standard for the research and quality control of buckwheat tea ,HPLC （high-performance liquid chromatography） was performed to de-termine quercetin and kaempferol in different kinds of buckwheat tea .The chromatographic separation was carried out on a DIKMA diamonsil （4.6 mm × 250 mm ,5 μm ） chromatographic column .The detection wavelength was set at 260 nm and the column temperature was 25 ℃ .The mobile phase was acetonitrile and 0.1% phosphoric acid solution at a flow rate of 1.0 mL/min .The results showed that of the samples studied black tarary buckwheat tea with the whole embryo had the lowest quercetin and kaempferol con-tents （0.704 ~ 1.089 mg/g） and that considerable differences existed between buckwheat tea samples of different origins .
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第10期200-204,共5页
Journal of Southwest University(Natural Science Edition)
基金
国家科技支撑计划项目(2012BAI27B07)
西南民族大学国家外国专家项目(2014)
