摘要
为了提高微藻的产量及油脂含量,本文采用自制鼓泡式柱状生物反应器,通过批式培养及两段法培养方式培养产油模式藻种-湛江等鞭金藻。考察了反应器中p H值、光强、叶绿素荧光、微藻生物量及其相关组分的变化,获取微藻生物反应器培养适宜的操作范围。结果表明,控制生物反应器上午8：00至晚上22：00 p H值为6.2~6.5,夜间22：00至次日8：00 p H值为7.0~7.5,入射光强为（10~50）μmol·m-2s-1,微藻的叶绿素荧光为0.68~0.58,培养7天后,反应器内藻细胞密度增加12倍,干藻粉油脂含量达35%,细胞生长动力学曲线符合Logistic方程,实现了富氮时微藻生物量的增长和缺氮时油脂的积累。该实验结果为生物反应器产油微藻培养工艺优化提供了依据。
In order to improve the biomass and oil content of microalgae,marine oleaginous microalgae Isochrysis zhanjianggensis as a model species was cultivated under batch culture conditions and a two-stage cultivation method in a self-made bubble column bioreactor. p H value, light intensity, chlorophyll fluorescence, microalgae biomass and related components were investigated to obtain the applicable operation range of the bioreactor. The results showed that p H value should be controlled between 6.2 to 6.5 from 8 a.m. to 10 p.m. and 7.0-7.5 from 10 p.m. to 8 a.m. of the next day, respectively, the incident light intensity should be controlled at（10-50） μmol·m-2s-1 and chlorophyll fluorescence should be controlled at 0.58-0.68. Under such conditions, the cell density of algae after 7 days＇ cultivation was 12-fold of that when inoculation, the oil content of dry algae was up to 35 %, and cell growth kinetics curves fitted the Logistic equation. The algal stain could accumulate the biomass under nitrogen-repletion conditions and accumulate the oil content under nitrogen-depletion conditions. The experimental results provide the basis for the cultivation process optimization of microalgae in bioreactors.
出处
《计算机与应用化学》
CAS
2015年第6期765-768,共4页
Computers and Applied Chemistry
基金
国家自然科学基金资助项目(50908028)
