摘要
在酶学领域中,超高压和温度类似,作为一种物理学方法,可以用来调节酶催化功能,促使酶的构象发生变化。对超高压调节酶催化功能的原理、影响酶构象的作用机制,以及一些新型研究方法等进行了综述。展望了此项新技术的发展前景。
The mechanisms of the catalytic activity regulations and the conformation changes of enzyme under high hydrostatic pressure were summarized in this paper. Different characterization of the behaviour of enzyme under high pressure was also reviewed. In enzymology, high hydrostatic pressure could be used to modulate the enzymatic activities and explore the conformational changes, which has attracted more and more attention. This review also discussed the future developments of this technique.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2015年第6期561-568,共8页
Journal of Food Science and Biotechnology
基金
教育部高等学校博士学科点专项科研基金项目(2013博导类)
关键词
超高压
酶催化
酶构象
分子机制
原位分析
high hydrostatic pressure
catalysis
conformation
mechanism
in-situ analysis