摘要
研究了高效液相色谱法(HPLC法)检测金银花凉茶饮料中绿原酸含量的方法。采用C18柱(4.6mm×250mm,5μm),在检测波长327nm、流动相乙腈-0.4%磷酸溶液(12:88)、流速1mL/min、进样量20μL、柱温30℃的色谱条件下进行试验研究。结果表明:金银花凉茶饮料中的绿原酸含量在O.4~102μg/mL范围内线性关系良好(r=0.9999),平均回收率为99.87%,RSD=1.44%(n=9),所建立的方法精密度、稳定性、重复性和准确度均良好,可用于金银花凉茶饮料中绿原酸含量的测定。
To study a method for determination of chlorogenic acid in honeysuckle tea herbal beverage with high performance liquid chromatography (HPLC). The optimal condition was contained that elite hypersil C18 (4.6 mm×250 mm, 5 μm), detection wavelength 327 nm, a mobile phase with acetonitrile -0.4% phosphoric acid solution (12:88), flow rate of 1 mL/min, column temperature 30 %. Chlorogenic acid in honeysuckle tea beverage showed a good linear relationship at a range of 0.4-102 μg/mL (r=0.9999). The average recovery rate was 99.87%. RSD=1.44% (n=9). The methods were high precision, high accuracy, good reproducibility, and high accuracy. The methods could be used to determine the chlorogenic acid in honeysuckle herbal tea beverage.
出处
《食品科技》
CAS
北大核心
2015年第6期348-351,共4页
Food Science and Technology
基金
重庆市教委科学技术研究项目(KJ1401402)
重庆第二师范学院创新团队计划(KYC-cxtd03-20141002)
关键词
金银花
凉茶
绿原酸
HPLC
honeysuckle
herbal tea
chlorogenic acid
HPLC