摘要
光学显微成像具有极为悠久的历史,但一直以来,光学成像一直受到衍射极限的限制而分辨率无法突破200 nm。近年来,超高分辨率显微技术的发展使得光学显微成像分辨率达到了20 nm以下。值得庆贺的是,德国科学家Stefan Hell、美国科学家Eric Betzig和William Moerner因其在超高分辨率显微技术方面的突出贡献获得了2014年的诺贝尔化学奖。在这篇文章中,我们就简要介绍一下超高分辨率显微技术的发展和应用,并带领读者一同寻访大师的科学足迹。
For over hundreds of years, resolution of optical microscopy was limited to 200 nm by the restric- tion of light barrier. But this barrier was broken by the promotion of super-resolution imaging techniques in the recent decade, resulting in a resolution below 20 nm. Recently, German scientist Stefan Hell and American scientists Eric Betzig and William Moerner have won the 2014 Nobel Prize for Chemistry by their works in super-resolution imaging. Here we will briefly introduce the progress and application of those techniques, and retrospect their routes to the splendid discovery.
出处
《生命的化学》
CAS
CSCD
2015年第3期430-437,共8页
Chemistry of Life
关键词
超高分辨率显微技术
可激活型荧光蛋白
诺贝尔化学奖
super-resolution microscopy
photoactivatable fluorescent protein
Nobel prize for Chemistry
