摘要
Porous silicon nanoparficles （pSiNPs） are a promising nanocarrier system for drug delivery owing to their biocompatibility, biodegradability, and non-inflammatory nature. Here, we investigate the fabrication and characterization of thermally hydrocarbonized pSiNPs （THCpSiNPs） and chitosan-coated THCpSiNPs for therapeutic oligonucleotide delivery. Chitosan coating after oligonucleotide loading significantly improves sustained oligonucleotide release and suppresses burst release effects. Moreover, cellular uptake, endocytosis, and cytotoxicity of oligonucleotide-loaded THCpSiNPs have been evaluated in vitro. Standard cell viability assays demonstrate that cells incubated with the NPs at a concentration of 0.1 mg/mL are 95% viable. In addition, chitosan coating significantly enhances the uptake of oligonucleotide-loaded THCpSiNPs across the cell membrane. Moreover, histopathological analysis of liver, kidney, spleen, and skin tissue collected from mice receiving NPs further demonstrates the biocompatible and non-inflammatory properties of the NPs as a gene delivery vehicle for intravenous and subcutaneous administration in vivo. Taken together, these results suggest that THCpSiNPs provide a versatile platform that could be used as efficient vehicles for the intracellular delivery of oligonucleotides for gene therapy.
多孔的硅 nanoparticles (pSiNPs ) 是为由于他们的 biocompatibility， biodegradability，和非煽动性的自然的药交货的一个有希望的 nanocarrier 系统。这里，我们调查制造和描述热地 hydrocarbonized pSiNPs (THCpSiNPs ) 和为治疗学的 oligonucleotide 的 chitosan 涂的 THCpSiNPs 交货。在显著地装载的 oligonucleotide 以后的 Chitosan 涂层改进持续 oligonucleotide 版本并且压制爆炸版本效果。而且，装载 oligonucleotide 的 THCpSiNPs 的细胞的举起， endocytosis，和 cytotoxicity 在 vitro 被评估了。标准房间生存能力试金证明在 0.1 mg/mL 的集中与 NP 孵化的房间是 95% 可行。另外， chitosan 涂层显著地越过房间膜提高装载 oligonucleotide 的 THCpSiNPs 的举起。而且，肝，肾，怒气，和皮组织的组织病理学说的分析从进一步收到 NP 的老鼠收集了在 vivo 为静脉内、下的管理作为基因交货车辆表明 biocompatible 和 NP 的非煽动性的性质。一起拿，这些结果建议 THCpSiNPs 提供能为基因治疗为 oligonucleotides 的细胞内部的交货被用作有效车辆的一个万用的平台。
