摘要
目的 探讨腹腔注射安钠咖对大鼠学习记忆能力及海马神经元形态的影响.方法 36只雄性SD大鼠按随机数字表法分为3组：对照组、安钠咖小剂量组及安钠咖大剂量组,每组12只,分别每天给药1次（腹腔注射l mL生理盐水或60 mg/kg安钠咖或120 mg/kg安钠咖）,连续20d.末次注射后次日应用Morris水迷宫实验（定位航行试验、空间探索试验、可见平台试验）评定各组大鼠的空间学习记忆能力,行为学实验结束后大鼠断头取脑、HE染色观察海马神经元形态的改变.结果 在定位航行试验中,安钠咖大剂量组各训练日的平均逃避潜伏期与对照组及安钠咖小剂量组相比差异均有统计学意义（P＜0.05）,而安钠咖小剂量组各训练日的平均逃避潜伏期与对照组比较差异均无统计学意义（P＞0.05）;在空间探索试验中,3组大鼠在目标象限内穿越原平台次数平均值比较差异无统计学意义（P＞0.05）.HE染色观察发现,安钠咖小剂量组大鼠除海马CA1区神经元细胞排列紊乱、稀疏外,其他部位与对照组相比无明显变化;而安钠咖大剂量组大鼠海马CA1区和齿状回区神经元细胞排列紊乱、核固缩深染、胞浆浓缩.结论 大剂量安钠咖可使大鼠学习能力下降,且会损害海马神经元.
Objective To observe the effect ofintraperitoneal injection of sodium benzoate on learning and memory abilities and morphology of hippocampal neurons in rats.Methods Thirty-six male SD rats were randomly divided into three groups：control group,sodium benzoate low-dose group and sodium benzoate high-dose group （n=12）;intraperitoneal injection of 1 mL normal saline,60 mg/kg sodium benzoate or 120 mg/kg sodium benzoate was performed on the rats of three groups,respectively,for 20 d.Morris water maze test （place navigation,space exploration and visible platform searching） was used to assess the spatial learning and memory of rats,and HE staining was used to observe morphological changes in hippocampal neurons.Results The escape latency of rats in the sodium benzoate high-dose group was statistically significant in the navigation test as compared with that in the control group and the sodium benzoate low-dose group （P〈0.05）;no significant difference was noted between sodium benzoate low-dose group and control group （P〉0.05）.In probe test,no significant differences in the number crossing target quadrant were found after withdrawal of platform between groups （P〉0.05）.HE staining showed that hippocampal CA1 neurons were sparse,showing a disordered arrangement in sodium benzoate low-dose group;the hippocampal neurons of CA1 region and fascia dentata region in sodium benzoate high dose group showed messed arrangement,nuclear pyknosis and cytoplasmic concentration as compared with that in the control group.Conclusion The high-dose sodium benzoate can decrease the learning ability of rats and impair hippocampal neurons.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2015年第5期464-468,共5页
Chinese Journal of Neuromedicine
基金
山西省卫生厅科技公关项目（2011114）
关键词
安钠咖
学习能力
记忆能力
海马
神经元
Sodium benzoate
Learning ability
Memory ability
Hippocampus
Neuron
