摘要
通过对一株高山被孢霉菌株(Mortierella alpina)发酵生产花生四烯酸(ARA)的摇瓶培养研究,以菌体干质量、出油率和ARA含量为标准,确定了其最佳发酵工艺条件。摇床实验确定的最佳培养条件为摇床转速180 r/min,培养基碳氮比为3.0,发酵温度为26℃,p H值为6.2,发酵周期为5 d。在此发酵条件下,在10 L自动发酵罐进行放大发酵试验,菌体干质量、出油率和ARA含量分别高达45.4 g/L、24.1 g/L和49.6%。
The optimal fermentation conditions ofMortierella alpina for arachidonic acid （ARA） production were studied from the indexes of dry cell mass, oil yield and ARA yield. The results showed the optimal condition was shaking speed 180 r/min, C/N ratio 3.0, fermentation temperature 26℃, pH 6.2 and cultivation time 5 d. Further study in 10 L fermentor was operated based on the optimal condition, the dry cell mass, oil yield and ARA production was 45.4 g/L, 24.1 g/L and 49.6%, respectively.
出处
《中国酿造》
CAS
北大核心
2015年第5期52-55,共4页
China Brewing
基金
国家高技术研究发展计划‘863计划’项目(2014AA021701
2015AA021003)
关键词
高山被孢霉
花生四烯酸
培养条件
放大发酵实验
Mortierella alpine
arachidonic acid
cultivation condition
scale-up fermentation
