摘要
为了将中草药作为饲料添加剂长期应用于虹鳟养殖生产,探讨了复方中草药对虹鳟Oncorhynchus mykiss抗氧化能力的影响。在水温为(17±1)℃条件下,将体质量为(200.0±1.5)g的1+龄虹鳟随机分为2个试验组和1个对照组,每组120尾鱼,每组设1个平行,饲养在室外循环水圆形水泥池(直径6 m)中。两个试验组鱼分别投喂添加茵陈、板蓝根等(复方一)和添加紫草、金银花等(复方二)的全价饲料,对照组鱼只投喂全价饲料,共饲养30 d。停饲24 h后,采集试验鱼的血清、肝脏、脾脏、肾脏,测定各组织中丙二醛(MDA)、一氧化氮(NO)含量,以及总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(i NOS)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX)的活性及总抗氧化能力(TAOC)。结果表明:两个试验组虹鳟肝脏中SOD活性均上升,MDA含量均显著低于对照组(P<0.05);复方一试验组肝、脾和肾脏中NO含量,脾脏中i NOS活性以及血清中T-AOC活性均显著高于对照组(P<0.05),血清中GSH-PX活性也相应提高;复方二试验组肾脏中NO含量显著高于对照组(P<0.05)。研究表明,复方一较复方二能更有效地提高虹鳟的抗氧化能力。
A feeding trial was conducted to evaluate the effects of two kinds of Chinese herbal compound on antioxidant defenses of rainbow trout Oncorhynchus mykiss for application of Chinese herbal compound in rainbow trout culture. Juvenile rainbow trout of 1＋old with body weight of（ 200. 0 ± 1. 5） g were randomly divided into outdoor circulating circular tanks at a rate of 120 fish per tank and fed basal diet and the diets containing two kinds of Chinese herbal medicine compounds（ No. 1 Artemmisia capillaries and Isatis indigotica; No. 2 Arnebia guttata and Lonicera japonic） at feeding rate of 2. 5% with duplication at water temperature of（ 17 ± 1） ℃ for 30 days. The NO and malonaldehyde（ MDA） contents,activities of total nitric oxide synthase（ TNOS）,inducible nitric oxide synthase（ i NOS）,superoxide dismutase（ SOD） and glutathione peroxidase（ GSH- PX） and total antioxidant capacity（ T- AOC） were determined in serum,liver,spleen and kidney of the rainbow trout fasted for 24 hours at the end of the experiment. The results showed that there were significantly higher activity of SOD and significantly lower content of MDA in liver in the rainbow trout fed the diets containing Chinese herbal medicines than those in the juveniles fed the basal diet（ P〈0. 05）. The contents of NO in liver,spleen and kidney,the activity of i NOS in spleen,and the serum T- AOC were found to be increased significantly in the rainbow trout fed the diets containing Chinese herbal compound 1（ P〈0. 05）,with higher activity of GSH- PX in serum. The rainbow trout fed the diets containing Chinese herbal compound 2 had significantly higher content of NO in the kidney. The findings indicate that compound 1 improves the antioxidant capacity effectively,and can be used as an excellent antioxidant of Chinese herbal medicine for rainbow trout.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2015年第2期170-174,共5页
Journal of Dalian Ocean University
基金
国家"十二五"科技支撑计划项目(2012BAD25B10)
关键词
虹鳟
中草药
抗氧化
Oncorhynchus mykiss
Chinese herbal medicine
antioxidant capacity
