摘要
目的以快速老化模型SAM小鼠的海马组织作为研究对象,通过miRNA芯片大规模筛选与衰老相关的miRNAs,为探讨衰老的机制提供线索。方法随机各选取3只4、8和12月龄SAMP8及SAMR1小鼠,提取海马组织RNA进行芯片检测;以snRNA U6作为内参,SYBR Green作为染料,用real-time PCR方法进行验证,对miRNA的表达进行相对定量分析。结果对比miRNA芯片和real-time PCR验证结果,证实芯片结果具有相对较好的重复性和真实度。对3张miRNA芯片结果进行综合分析,在4和8月龄均表现出表达差异的miRNA共7个,在8和12月龄均表现出表达差异的miRNA共8个,4和12月龄均表现出表达差异的miRNA共3个,在3个月龄间均表现出明显表达差异的1个(miR-9*)。结论 miRNAs在SAMP8及SAMR1的差异表达提示其在SAMP8衰老进程中发挥重要作用。
Objective To explore the mechanisms of aging,hippocampal tissue of senescence accelerated mouse（ SAM） were used to screen aging related miRNAs. Methods SAMP8 and SAMR1 mice（ 4-,8-,12-month old）were randomly selected 3 in each group,extracting RNA from hippocampal tissue for miRNA microarray. To verify the relative quantitative expression of miRNAs,SYBR Green was used as a dye for the real-time PCR and snRNA U6 was used as control. Results Based on the validation of real-time PCR,here we present the microarray results which have better repeatability and authenticity. Comprehensive analysis of three repeat miRNA array data showed that 7 miRNAs,8 miRNAs and 3 miRNAs were dynamically expressed in the 4- month /8- month,8- month /12-month and 4- month /12- month hippocampal tissues,respectively. Only miR-9＊showed an obviously differential expression pattern in both the three stages. Conclusions The differentially expressed miRNAs in SAMP8 andSAMR1 mice could play an important role in the aging process.
出处
《基础医学与临床》
CSCD
2015年第5期674-679,共6页
Basic and Clinical Medicine
基金
重大科学研究计划(2011CBA01104)
关键词
衰老
MIRNAS
SAM小鼠
海马
aging
miRNAs
senescence accelerated mouse
hippocampus
