摘要
目的 观察急性肺栓塞大鼠血小板活化标志物GMP-140和CD63的表达及动态变化并探讨其对肺栓塞的诊断价值.方法 健康雄性Wistar大鼠40只,随机分为4组：对照组、栓塞后6h组、栓塞后12 h组、栓塞后24 h组,每组10只.将栓子经颈静脉注入,制备大鼠急性肺栓塞模型,对照组注射等量生理盐水.在相应时间点采血分离富含血小板血浆,应用流式细胞术检测GMP-140和CD63的表达.结果 血小板活化标志物GMP-140在栓塞后6h、12 h、24 h组中表达阳性率分别为（2.031±0.417）％、（1.311±0.387）％、（0.929±0.244）％,均高于对照组[（0.538士0.341）％,P＜0.01];血小板活化标志物CD63在栓塞后6h、12 h、24 h组中表达阳性率分别为（2.642±1.394）％、（1.850±0.982）％、（0.887±0.290）％,均高于对照组[（0.404±0.305）％,P＜0.01];均以栓塞后6h表达最显著.其中GMP-140的表达栓塞后6h组同栓塞后12h、24 h组比较差异有统计学意义（P＜0.01）,栓塞后12 h组与栓塞后24 h组间比较差异无统计学意义（P＞0.01）;CD63的表达栓塞后12h组同栓塞后24 h组间比较差异无统计学意义（P＞0.01）,栓塞后6h组与栓塞后24 h组间比较差异有统计学意义（P＜0.01）.结论 实验组大鼠不同时间GMP-140和CD63均升高,可以为肺栓塞的诊断提供依据.
Objective To observe the expressions and the dynamic change of platelet activation markers GMP140 and CD63 in rats with acute pulmonary embolism,so that to explore the value of the markers in early diagnosis and treatment of pulmonary embolism.Methods Forty healthy male Wistar rats were divided randomly into four groups as follows：six hours after embolism group,12 hours after embolism group,24 hours after embolism group,and control group.Each group contained ten rats.Emboli was injected through the jugular vein to establish the model of pulmonary embolism,while the same amount of normal saline was injected through the jugular vein to the control group.The platelet-rich plasma was obtained from blood sample collected from experimental rats and control rats.The expressions of platelet-associated GMP-140 and CD63 in platelet-rich plasma were detected by flow cytometry.Results The expression of platelet activation marker GMP-140 in six hours,12 hours,24 hours after embllism groups [（2.031±0.417）%,（1.311±0.387）%,（0.929±0.244）%] was higher than that in control group [（0.538± 0.341）%,P 〈0.01].The expression of platelet activation marker CD63 in six hours,12 hours,24 hours after embllism groups [（2.642 ± 1.394） %,（1.850 ± 0.982） %,（0.887 ± 0.290）%] was higher than that in control group [（0.404±0.305）%,P 〈0.01].The expressions of the markers were most significant in six hours after embolism group.The expression of GMP-140 in six hours group was significant higher than that in 12 hours group and 24 hours group （P 〈0.01）,while there was no difference in the expression of GMP-140 and CD63 between 12 hours group and 24 hours group （P 〉 0.01）.There was statistical difference in the expression of CD63 between six hours group and 24 hours group （P 〈0.01）.Conclusions The expressions of GMP-140 and CD63 increase at different time points of the experimental groups.The results can provide reference for the assess of pulmonary thromboembolism.
出处
《国际呼吸杂志》
2015年第8期581-585,共5页
International Journal of Respiration
关键词
肺血栓栓塞
动物模型
血小板活化标志物
Pulmonary thromboembolism
Animal model
Platelet activation marker
