摘要
[目的]探明Cry1Ab蛋白通过培养基-果蝇-拟环纹豹蛛这一食物链的传递,最终在拟环纹豹蛛（Pardosa pseudoannulata）体内的富集情况以及毒蛋白随食物链的传递在各营养级中的变化规律。[方法]采用酶联免疫技术（ELISA）测定Cry1Ab蛋白在拟环纹豹蛛和果蝇中的含量。[结果]Cry1Ab蛋白对不同龄期拟环纹豹蛛的体长、背甲宽、中眼域宽、腿长等生长指标不存在显著性差异,但对体重产生一定的影响,使得其体重略有下降的趋势。在0-10 d Cry1Ab蛋白在拟环纹豹蛛体内不断累积,但10 d后拟环纹豹蛛体内Cry1Ab蛋白含量逐渐降低。[结论]Cry1Ab蛋白在拟环纹豹蛛体内具有富集效应。
[ Objective ] The aim was to prove food chain transfer of Cry1 Ab protein through the medium - fruit flies and intends to P. pseudoannulata, the final accumulate in P. pseudoannulata body as well as toxic protein variation in each trophic level with the transfer of the food chain. [ Method] Using enzyme-linked immunosorbent assay （ELISA） the content of Cry1Ab protein in the P. pseudoannulata and Drosophila melanogaster was determined. [ Result]The results showed that,there was no signifant difference that Cry1Ab protein on body length,carapace width,the width of the eye domain,legs and other growth indicators of P. pseudoannulata in different ages, but had a certain impact on body weight, so its weight was slightly a downward trend. Cry1Ab protein within 0 -10 d accumulated in P. pseudoannulata body,but after 10 days Cry1Ab protein content intended to decrease. [ Conclusion] The study had shown the Cry1Ab protein enrichment effect of P. pseudoannulata body.
出处
《安徽农业科学》
CAS
2015年第14期13-15,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(31472017)
湖南省科技厅重点项目(2014FJ2003)
关键词
拟环纹豹蛛
果蝇
Cry1Ab蛋白
食物链
Pardosa pseudoannulata
Drosophila melanogaster
Cry1 Ab protein
Food chain
