期刊文献+

基于多孔普鲁士蓝/金复合材料的酶电化学传感器的研究 被引量:2

Microporous Prussian Blue / gold composite material based enzyme electrochemical sensor
在线阅读 下载PDF
导出
摘要 以氢气泡为模板,利用电化学沉积等手段制备微/纳米多孔普鲁士蓝/金复合材料.通过化学修饰将葡萄糖氧化酶固定于复合材料表面,构建具有高灵敏度的电化学葡萄糖传感器,并采用计时电流法研究该传感器对葡萄糖的电化学催化活性.结果表明,该电化学传感器对葡萄糖的响应速度为7 s,检测线性范围为0.05~ 3.75 mmol/L.此外,该酶传感器还具有良好的重现性和稳定性. Microporous Prussian Blue/gold composite material was prepared with electrochemical deposition and hydrogen bubble as a template. We designeda highly sensitive electrochemical glucose sensor through immobilizing glucose oxidase on the surface of the composite material. We further employed typical current-time response to investigate its electro-catalytic property to glucose. Results show that its response time is 7 s, and linear detection range is O. 05 to 3.75 mmol/L. The sensor also has better reproducibility and higher stability.
作者 王春蕾 林猛
出处 《山东科学》 CAS 2015年第2期53-57,共5页 Shandong Science
关键词 普鲁士蓝 葡萄糖 微孔金电极 生物传感器 Prussian Blue glucose microporous gold electrode biosensor
  • 相关文献

参考文献15

  • 1CLARK L C Jr, LYONS C. Electrode systems for continuous monitoring in cardiovascular surgery [ J ]. Annals of the New York Academy of Sciences, 1962, 102:29-45.
  • 2WANG J. Electrochemical glucose biosensors [J]. Chem Rev, 2008, 108(2): 814-825.
  • 3KAUSHIK A, KHAN R, SOLANKI P R, et al. Iron oxide nanopartieles-chitosan composite based glucose biosensor [ Jl. Biosens Bioelectron, 2008, 24(4): 676-683.
  • 4YANG J, LIN M, CHO M, et al. Determination of hydrogen peroxide using a Prussian Blue modified macroporous gold electrode [ J ]. Microchim Acta, DOI : 10. 1007/s00604 - 014 - 1433 - 0.
  • 5HUANG X J, LI C C, GU B, et al. Controlled molecularly mediated assembly of gold nanooctahedra for a glucose biosensor [ J]. J Phys Chem C, 2008, 112(10) : 3605 -3611.
  • 6ZHOU K F, ZHU Y H, YANG X L, et al. Electrocatalytic oxidation of glucose by the glucose oxidase immobilized in graphene- Au-Nafion biocomposite [ J ]. Electroanal, 2010, 22 (3) : 259 - 264.
  • 7LAUREYN W, SARA M, FRIEDT J M, et al. Realization and characterization of porous gold for increased protein coverage on aeoustic sensors [ J]. Anal Chem, 2004(15) : 4299 -4306.
  • 8YIN H M, ZHOU C Q, XU C X,et al. Aerobie oxidation of D-glucose on support-free nanoporous gold [ J ]. J Phys Chem C, 2008, 112:9673-9678.
  • 9LIU Z, SEARSON P C. Single nanoporous gold nanowire sensors [J]. J Phys Chem B, 2006, 110(9) : 4318 -4322.
  • 10WANG X, LIU X Y, YAN X L, et al. Enzyme-nanoporous gold biocomposite: excellent biocatalyst with improved biocatalytic performance and stability [J]. PLoS ONE, 2011, 6(9) : e24207.

二级参考文献14

  • 1Arduini F,Cassisi A,Amine A,et al. Electrocatalytic oxida-tion of thiocholine at chemically modified cobalt hexacyano-ferrate screen 一 printed electrodes [ J]. J Electroanal Chem,2009,626(1 -2) : 66 -74.
  • 2Abbaspour A, Khajehzadeh A, Ghaffarinejad A. Electrocata-lytic oxidation and determination of hydrazine on nickelhexacyanoferrate nanoparticles - modified carbon ceramic e-lectrode[ J]. J Electroanal Chem,2009,631 (1 -2) : 52 -57.
  • 3Benesetto G E D, Cataldi T R I. Highly - stabilized polynu-clear indium - hexacyanoferrate film electrodes modified byruthenium species [ J]. Langmuir, 1998,14 ( 21 ) : 6274 -6279.
  • 4Lange U, Roznyatovskaya N V, Mirsky V M. Conductingpolymers in chemical sensors and arrays [ J]. Anal ChimActa,2008,614 (1) : 1-26.
  • 5Witkowski A,Freund M S, Brajtertoth A. Effect of electrodesubstrate on the morphology and selectivity of overoxidizedpolypyrrole films [ J]. Anal Chem, 1991, 63 ( 6 ) ; 622 -626.
  • 6Jiang X H,Lin X Q. Overoxidized polypyrrole film directedDNA immobilization for construction of electrochemical mi-cro -biosensors and simultaneous determination of serotoninand dopamine[ J]. Anal Chim Acta,2005,537( 1 -2) ; 145-151.
  • 7Fernandez I, Trueba M, Nuez C A. Some features of theoveroxidation of polypyrrole synthesized on austenitic stain-less steel electrodes in aqueous nitrate solutions [ J]. SurfCoat Technol,2005,191(1) : 134 -139.
  • 8Tsionsky M,Gun J,Glezer V, et al. Sol - gel - derived ce-ramic -carbon composite electrodes : introduction andscope of applications [ J]. Anal Chem,1994,66 (10):1747 -1753.
  • 9Laviron E. General expression of the linear potential sweepvoltammogram in the case of diffusionless electrochemicalsystems[ J]. J Electroanal Chem, 1979,101 ( 1) : 19 -28.
  • 10Bard A J,Faulkner L R,Electrochemical methods: funda-mentals and applications [ M]. 2nd edition, New York:Wiley, 2001,163.

共引文献1

同被引文献12

引证文献2

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部