摘要
以大果沙棘浆果黄酮(ZYCFJ)为参照,考察了张掖大果沙棘黄酮(ZYCF)和新疆野生沙棘枝叶黄酮(XJCF)的抗氧化活性。结果表明:沙棘浆果黄酮和沙棘枝叶黄酮对不同自由基的清除效果均有差异。在黄酮浓度为50μg/m L时,ZYCFJ、ZYCF和XJCF对ABTS+清除率分别为60.8%、42.6%、45.4%;对DPPH-的清除率分别为95.7%、80.6%、88.6%,在黄酮浓度高于40μg/m L时,ZYCFJ对DPPH-和ABTS+的清除效果比ZYCF和XJCF的好。在黄酮浓度为500μg/m L时,ZYCFJ、ZYCF和XJCF对·OH的清除率分别为92.7%、88.9%、98.6%;对O-·2的清除率分别为76.5%、70.6%、82.4%;对亚硝酸盐的清除率分别为98.7%、76.8%、83.7%。就对亚硝酸盐的抑制和Fe3+的还原来说,ZYCFJ明显优于ZYCF和XJCF;对超氧阴离子和羟自由基的清除效果ZYCFJ、ZYCF和XJCF的相当。
Uslng flavonoids from Hippophae rharnrtoides L. berry （ZYCFJ） as reference,the antioxidant activities of fla- vonoids in branches and leaves of Hippophae rhanmoldes L. from Zhangye （ZYCF） and Xinjiang （XJCF） were evaluated by measuring their scavenging activity against DPPH,ABTS,hydroxyl,superoxide anion radicals and reducing Fe^3 ＋ and inhibiting nitroso. The results showed that at 50 μg/mL concentration level, the clearance rates of ZYCFJ, ZYCF and XJCF on ABTS^ ＋ were 60.8% ,42.6% ,45.4%, and on DPPH＇were 95.7% ,80.6%. 88.6%. When flavonoids concentrations higher than 40 μg/mL, the scavenging activity against DPPH and ABTS ^＋ of ZYCFJ was better than XJCF and ZYCF. At 500 μg/mL concentration level,the scaveng/ng rates of ZYCFJ,ZYCF and XJCF on · OH were 92.7%, 88. 9% ,98.6% ,and on O^2-were 76.5% ,70.6% ,82.4% ,and inhibition ratio of ZYCFJ, ZYCF and XJCF on nitrite were 98.7% ,76.8% ,83.7%. In term of the inhibition rates of nitrite and the reduction of Fe^3＋ ,ZYCFJ was better than that of ZYCF and XJCF. The scavenging effect of ZYCFJ,ZYCF and XJCF on superoxide anion and hydroxyl free radical were equivalent.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2015年第4期655-660,共6页
Natural Product Research and Development
基金
国家中医药管理局2012年中医药行业科研专项(201207002)
甘肃省高校河西走廊特色资源利用省级重点实验室科研资助项目(XZ12-2)
关键词
沙棘枝叶
黄酮
张掖大果沙棘
新疆野生沙棘
抗氧化性
Hippophae rhamnoides L. branch
flavonoids
Hippophae rhamnokies L. from Zhangye
Hippophae rhamnoides L. from Xinjiang
antioxidant activity
