摘要
已有证据表明，microRNA在动物皮毛发育和形态发生过程中发挥重要的作用。在山羊皮肤microRNA的研究中，数据库不完善仍是限制其功能研究的主要原因。本研究以山羊基因组为参考，对高通量测序中未注释的占总reads 5．40％的数据，使用Mireap软件预测的方法进行数据挖掘，并对新的microRNA的长度、表达量、基因组定位、靶基因预测及KEGGpathway进行分析。研究预测到28个新的microRNA，其长度分布与已知山羊、牛等动物的分布一致。对表达量统计表明，表达量在100以上的有4个，最高的是Novel-15，达到555；表达量在10～100的有19个；低于10的有5个。基因组定位研究表明，新microRNA在山羊染色体上分布均匀，在5、6、7、19和X染色体上分别有两个microRNA被定位。预测和分析得到6519个靶基因。KEGG分析结果表明，有3552个靶基因定位到51条通路中，占前3位的通路是Metabolic pathways、Pathways in cancer、MAPK signaling pathway。本研究发掘了更多山羊的microRNA，为今后山羊皮肤发育调控的研究奠定了基础。
microRNA played an important role in the process of the animals skin development and their morphogenetic process in previous studies. The database imperfect was still the main reason for limiting its function studies in the study about the microRNA from the goat skin. Our research analyzed the 5.40% no annotation reads from the high-throughput sequencing total reads,reference the goat genomics gene, using the Mireap software to analyze the data,and analyzed the data including new microRNA length, expression, genomics location, predict the target gene and the KEGG pathway. Our research got 28 new microRNA,and their length were equal with the known microRNA in the goat and the cow. Their expression statistics showed that, there were 4 new microRNA their expression over the 100, the highest was the Novel-15,its expression got 555; There were 19 new microRNAs their expression between 10 to 100 ;There were 5 new microRNA their expression lower 10. We found the new microRNA evenly distributed over the goat chromosome by studying genomics location,exactly locate 2 microRNA on the number 5,6,7,19 and X chromosome respectively. Predicted and analyzed the target gene data we got 6 519 target gene. Analyzed the KEGG pathway showed that,locate the 3 552 target gene on the 51 pathway, the three former pathway were Metabolic pathways,Pathways in cancer and MAPK signaling path- way. Our research found more new goat microRNA, had laid the foundation about the study of the goat skin development regulate.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第4期775-781,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家863计划(2013AA102506)
国家自然科学基金(31360537
31272421)
内蒙古自然科学基金(2013MS0411)
教育部博士点基金(20121515120009)
