摘要
目的:在细胞水平探索mi R-21在调控TGF-β1诱导的大鼠骨髓间充质干细胞向肌成纤维细胞分化中的作用。方法:全髓培养法培养原代大鼠骨髓间充质干细胞,培养至第三代时用TGF-β1分组诱导培养,检测TGF-β1对大鼠BMSCs促纤维化的作用及该过程中不同浓度梯度TGF-β1诱导以及不同时间段mi R-21的表达变化;通过转染mi R-21 mimics(高表达)不同时间点检测其对α-SMA表达的影响。结果:TGF-β1能促进大鼠骨髓间充质干细胞向成纤维,肌成纤维细胞分化;大鼠骨髓间充质干细胞向成纤维,肌成纤维细胞分化后mi R-21表达上调;上调mi R-21能促进大鼠BMSCs的纤维化作用。结论:mi R-21 mimics能够促进大鼠BMSCs的纤维化作用。
Objective：To investigate the role of miR-21 in the jaw of fibrosis in vitro. Method：Whole bone marrow adherence on plastic was used to isolation and culture rat mesenchymal stem cells. When passage 3, the cells were treated with TGF-β1 for different periods of time and concentration. The target gene expression of miR-21 was detected by real-time PCR;Over-expression of miR-21 by niR-21 mimics,to study whether up-regulated miR-21 enhances myfibroblast differentiation of rat mesenchymal stem cells by immunohistochemistry. Result：Real-time PCR demonstrated that TGF-β1 induced miR-21 expression in a time-dependent and dosage-dependent manner,peaking at 48h with an optimal dose at 5 ng/mL. The protein expression of α-SMA was up-regulated. Conclusion：miR-21 could promote myfibroblast differentia-tion of BMSCs in vitro. Inhibition of miR-21 may be a therapeutic approach to suppress renal fibrosis.
出处
《临床口腔医学杂志》
2015年第4期213-216,共4页
Journal of Clinical Stomatology
基金
国家自然科学基金(NSFC:81271112)
上海市人才发展资助项目(201312)
上海交通大学晨星计划(a)
