摘要
目的 应用CEUS技术观察兔肝VX2肿瘤坏死过程, 探讨VX2肿瘤生长坏死规律及其对实验产生的影响。方法 应用超声引导下经皮穿刺种植法成功建立24只兔VX2肝肿瘤模型, 并通过CEUS技术分别在肿瘤种植后10、14、21、28天动态观察肝肿瘤演变过程, 测量肿瘤体积及坏死体积, 计算瘤内坏死率。结果 在肿瘤种植后10、14、21、28天兔肝VX2肿瘤体积、坏死灶体积和坏死率比较, 差异均有统计学意义（P均〈0.05）;肿瘤种植后10、14、21、28天的 CEUS表现能反映出肿瘤的病理学特征。实验依据CEUS和病理特点将肿瘤生长过程细分为5个时期,0-10天为肿瘤实质前期;11-14 天为肿瘤实质后期;15-21天为肿瘤坏死初期;22-28天为加速坏死期;28天后为肿瘤囊变期。结论 CEUS可准确判断肝肿瘤实质及坏死范围;VX2肿瘤的生长演变及肿瘤坏死具有规律性, 可根据不同实验要求选择适宜的研究时间段。
Objective To observe the rabbit VX2 liver tumor necrosis process with the CEUS, and to explore the pattern of VX2 tumor growth and necrosis. Methods Twenty-four rabbits were used to establish VX2 liver tumor models with the guidance of ultrasound, and CEUS were performed on 10th, 14th, 21th, 28th days. The size of liver tumor parenchyma and necrosis were measured, and the tumor necrosis percentage was calculated. Results The size of the rabbit VX2 liver tumor, the focal necrosis and the tumor necrosis percentage had obvious difference （all P 〈0.05） on 10th, 14th, 21th and 28th days. The characteristics of tumor in CEUS were consistent with pathology features. According to the CEUS and pathological characteristics, tumor growth process were divided into 5 periods： 0—10 days as early tumor parenchyma stage; 11—14 days as later tumor parenchyma stage; 15—21 days as early tumor necrosis stage; 22—28 days as accelerated necrosis stage; after 28th day as cystic changes stage. Conclusion CEUS can judge the scope of liver tumor parenchyma and necrosis accurately. And the growth of VX2 tumor parenchyma and necrosis is regularity. It is useful to select appropriate research time according to different EU experimental requirements.
出处
《中国介入影像与治疗学》
CSCD
北大核心
2015年第4期238-241,共4页
Chinese Journal of Interventional Imaging and Therapy
基金
国家自然科学基金(81171355)
关键词
超声检查
肝肿瘤
兔
坏死
Ultrasonography
Liver neoplasmas
Rabbits
Necrosis
