摘要
为确定大黄鱼(Larimichthys croceus)对饲料硒的需求量,以Na2Se O3为饲料硒源,配制6种饲料,硒的添加水平分别为0(对照组)、0.05、0.2、0.4、0.6和0.9 mg/kg,实测值分别为0.08、0.16、0.27、0.44、0.66和0.96 mg/kg。在海水浮式网箱中养殖初始体重为(9.14±0.09)g的大黄鱼幼鱼10周,结果表明增重率(WG)、全鱼和骨骼中的硒含量随着饲料硒含量的升高而显著升高(P<0.05)。当饲料硒含量分别超过0.27、0.66、0.66 mg/kg时,这些指标的变化趋于平稳。饲料硒含量对存活率(SR)、饲料效率(FE)、体组成、肝体比(HSI)、脏体比(VSI)和肥满度(CF)都没有显著影响(P>0.05)。在血清中谷胱甘肽过氧化物酶(GPX)活性、超氧化物歧化酶(SOD)活性和总抗氧化力(T-AOC)随着饲料硒含量的升高呈现先升高后稳定的趋势(P<0.05),并分别在饲料硒含量为0.44、0.44、0.16 mg/kg时达到最大值。肝脏中GPX活性、SOD活性、T-AOC、过氧化氢酶(CAT)活性和谷胱甘肽还原酶(GR)活性与血清中相应酶的活性有相同的趋势。在肝脏中谷胱甘肽硫转移酶(GST)活性随着饲料硒含量的升高呈现先降低后升高的趋势(P<0.05),并在饲料硒含量最高(0.96 mg/kg)时其活力取得最大值。以WG为评价指标,得出大黄鱼幼鱼对饲料中硒的需求量为0.178 mg/kg。以全鱼和骨骼中硒含量、肝脏GPX活性为评价指标,得出大黄鱼幼鱼对饲料中硒的最小需求量分别为0.575、0.387和0.440 mg/kg。
To study the effects of selenium to the juvenile large yellow croaker Larimichthys croceus, six experimental diets were formulated to contain the following diets with increment levels of selenium 0 （control）, 0.05, 0.2, 0.4, 0.6 and 0.9 mg/kg, respectively. The final content of selenium in each diet was 0.08, 0.16, 0.27, 0.44, 0.66, and 0.96 mg/kg, respectively. Each diet was randomly assigned to triplicate groups of 60 large yellow croaker juveniles [initial body weight：（9.14 ± 0.09） g]. Fish were fed twice daily （5：00 and 17：30） to satiation for 10 weeks. The results showed that the weight gain rate （WG）, whole-body, and vertebrae Se concentration significantly increased with the increased dietary selenium levels （P＆lt;0.05）, and no further increases when the dietary Se concentration was 0.27 mg/kg or higher in the diet. No significant differences were found in the survival rate （SR）, feed efficiency （FE）, body compositions, hepato-somatic index （HSI）, viscerosomatic index （VSI）, and condition factor （CF） cross the 6 treatment groups （P〉0.05）. The serum glutathione peroxidase （GPX） activity, superoxide dismutase （SOD） activity and total antioxidant capacity （T-AOC） significantly increased with the increasing of dietary selenium levels （P＆lt;0.05） with the highest activities at 0.44 mg/kg and 0.16 mg/kg of dietary selenium, respectively. The GPX activity, SOD activity, T-AOC, catalase （CAT） activity, and glutathione reductase （GR） activity in liver had a similar trend with serum enzyme activity. The liver glu-tathione S-transferase （GST） activity significantly decreased with the increasing of dietary selenium levels （P〈0.05）, and then increased （P〈0.05）. The highest activity was found in the highest Se level （0.96 mg/kg）. Based on WG, the dietary Se requirement of juvenile large yellow croaker was 0.178 mg/kg. Based on the whole-body and vertebrae Cu concentration, liver GPX activity, the minimum dietary Se requirements were 0.575, 0.387 and 0.440 mg/kg, respec-tively.
出处
《水生生物学报》
CAS
CSCD
北大核心
2015年第2期241-249,共9页
Acta Hydrobiologica Sinica
基金
国家公益性行业(农业)科研专项经费项目(编号:201003020,200903029)资助
关键词
大黄鱼
硒
生长
抗氧化
需求量
Large yellow croaker
Selenium
Growth
Antioxidation
Requirement
