摘要
目的:探讨缺氧条件下Galectin-1对口腔鳞癌细胞Tca8113的生物学行为的影响。方法:siRNA干扰Galectin-1在Tca8113细胞中的表达;采用MTT法检测细胞的增殖;Real time-PCR分析Galectin-1 mRNA和Bcl-2 mRNA的表达;Transwell小室观察各组细胞的侵袭能力;免疫荧光分析缺氧条件下细胞的凋亡情况。结果:siRNA-Galectin-l干扰组和200μmol/L氯化钴处理组的细胞出现了增殖抑制;用200μmol/L氯化钴处理Tca8113细胞后,Galectin-l mRNA的表达量上调(0.91±0.11,P<0.05);siRNA+Tca8113细胞组Bcl-2 mRNA的表达量明显减低(0.16±0.02,P<0.05);siRNA-Galectin-l干扰组迁移出的细胞数(31±6)明显低于200μmol/L氯化钴+Tca8113细胞组(43±6)和Tca8113细胞组(51±8,P<0.05);免疫荧光结果所示,siRNA+200μmol/L氯化钴+Tca8113细胞组的荧光强度最弱。结论:Galectin-1是反应肿瘤细胞缺氧的一个较为敏感的指标,可利用Galectin-1作为内在的缺氧标志物。
Objective:The biological effects of Galectin-l for oral squamous cell carcinoma cells in hypoxic environment were investigated. Methods:To interfere the expression of Galectin- 1 in cells with siRNA,the proliferation of cells was detected by MTT methods;the expression of Galectin- 1 mRNA and Bcl- 2 mRNA were analysed by real time- PCR,using Transwell to detect the invasion ability;to analyse the condition of cell apoptosis in hypoxic by immunofluorescence. Results:siRNA- Galectin- l group and 200μmol /L CoCl2 group appeared the inhibition of proliferation,the expression of Galectin- 1 mRNA was significantly increased in 200μmol /L CoCl2group(0. 91 ± 0. 11,P< 0. 05),the expression of Bcl- 2 mRNA was significantly reduced in siRNA group(0. 16 ± 0. 02,P < 0. 05);the cell counts of siRNA- Galectin- l interference group(31 ± 6) was significantly lower than 200μmol /L CoCl2group(43 ±6) was Tca8113 cell group(51 ±8,P <0.05),the fluorescence intensity of siRNA and 200μmol/L CoCl2 and Tca8113 cell group were the weakest. Conclusion:Galectin- 1 was a sensitive indicator with tumor cells in hypoxic environment,Galectin- 1 maybe used as an inner oxygen marker.
出处
《现代肿瘤医学》
CAS
2014年第9期2006-2009,共4页
Journal of Modern Oncology
基金
辽宁省教育厅高等学校科研计划项目(No:LS2010170)
辽宁省教育厅高校重点实验室支持计划项目(No:2009S112)
