摘要
目的:观察100 Hz电针刺激对脑组织和脾脏乙酰胆碱酯酶mRNA表达的影响,初步探讨电针的免疫功能调节作用机制。方法:将昆明小鼠24只随机分成对照组、插针不电组和电针组。给予小鼠电针4天后处死,用RT-PCR方法分别检测小鼠血清、延髓、下丘脑和脾脏中乙酰胆碱酯酶(ACh E)mRNA表达情况。结果:RT-PCR显示,小鼠血清、延髓、下丘脑和脾脏中均有乙酰胆碱酯酶mRNA的表达。结论:胆碱能抗炎通路可能参与了100 Hz电针调节免疫功能的作用。
Objective：To observe the effect of 100 Hz electro-acupuncture on the mRNA expressions of AChE and to in-vestigate the immunoregulation effect and mechanism of electro-acupuncture treatment.Methods：24 male KM rats were randomly divided into normal control group,non-electro acupuncture group,and electro-acupuncture group with 8 in each group.On the fourth day of electro-acupuncture,the rats were sacrificed and RNA was extracted by kit from the serum, medulla,hypothalamus,and spleen to detect the expressions of AChE by RT-PCR.Result：RT-PCR showed that AChE was expressed in serum,medulla,hypothalamus,and spleen.Conclusion：100 Hz electroacupuncture may regulate im-mune function via cholinergic anti-inflammatory pathway.
出处
《赣南医学院学报》
2015年第1期21-23,165,共3页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
国家自然科学基金项目(31060144)
江西省自然科学基金项目(2008GZY0067)资助
关键词
电针
免疫调节
胆碱能系统
乙酰胆碱酯酶
electro-acupuncture
immunoregulation
cholinergic system
acetycholinesterase （ AChE ）
