摘要
恶性疟原虫 DNA 与 pBR_(322)质粒 DNA 体外重组后,导入 E.coli HB_(101)建成基因文库,用疟原虫全基因组 DNA 从文库中筛出了5个杂交信号特强的克隆 pBF_1,pBF_2,pBF_3,pBF_4和 pBF_5。克隆 pBF_4 DNA与 P.c DNA,P.b DNA,及人白细胞 DNA 均无交叉反应,与 P.f DNA 杂交时,可检出的最低 DNA 量为10pg。
Plasmodium falciparum genomic DNA library was built by parasite genomic fragments cloned in the plas- mid pBR_(322) which were digested with Hind Ⅲ.The clones were screened by hybridization in situ with whole genomic DNA and those giving the strongest signals have been selected.Among them,clone pBF_4 was chosen and analysed from the point of view of its specificity and sensitivity.pBF_4 DNA was digested by EcoRI, Hind Ⅲ,PstⅠ,SalⅠ and BamHI and showed a single insert of 3Kb.By dot blot analysis.the pBF_4 DNA hy- bridized strongly to P.falciparum genomic DNA but not to human DNA,P.cynomolgi DNA,P.bergei DNA.The results proved that the minimum amount of parasite DNA that can be detected by ^(32)P—labelled pBF_4 DNA after autohybridization is 10pg.
出处
《中国寄生虫病防治杂志》
CSCD
1991年第4期255-257,共3页
Chinese Journal of Parasitic Disease Control
