摘要
目的通过分析不同病毒载量慢性乙型肝炎（CHB）患者和乙型肝炎病毒携带者（ASC）外周血T淋巴细胞各亚群变化的规律,探讨乙肝病毒复制水平对T淋巴细胞免疫功能的影响及其可能机制。方法选择兰州市第二人民医院2012年7~12月收治的CHB患者63例（CHB组）,选取同期ASC患者112例（ASC组）,健康体检者84例（对照组）。采用流式细胞仪检测三项分组血清中T淋巴细胞亚群水平,实时荧光定量法检测HBV DNA水平。结果 CHB组患者和ASC组患者外周血CD3＋、CD4＋及CD4＋/CD8＋水平均低于对照组,差异有统计学意义（P〈0.05）;而CD8＋水平则显著高于对照组,差异有统计学意义（P〈0.05）。结论兰州地区慢性HBV感染者T淋巴细胞亚群存在不同程度免疫调节异常和功能障碍。
Objective To investigate the influence of hepatitis B virus （HBV ） replication level on the T lymphocyte immune function and its possible mechanism by analyzing the change rule of peripheral blood T lymphocyte subsets in the different HBV load patients with chronic hepatitis B （CHB） and asymptomatic hepatitis B virus carriers（ASC） .Methods 63 cases of CHB（CHB group group） ,112 cases of ASC（ASC group） and 84 individuals of physical examination（control group） in our hospital from July to December 2012 were selected and detected the various T lymphocyte subsets by flow cytometry and the HBV DNA level by the real time fluorescence quantitative polymerase chain reaction .Results The levels of peripheral blood CD3 ＋ ,CD4 ＋ lymphocyte cells and CD4 ＋ /CD8 ＋ T in the CHB group and ASC group were significantly lower than those in the control group ,the difference was statis‐tically significant （P〈0 .05） ;whereas the CD8 ＋ T level was obviously higher than that in the control group with statistical differ‐ence （P〈0 .05） .Conclusion Different degrees of immune dysregulation and immune dysfunction exist among the patients with chronic HBV infection in Lanzhou area .
出处
《国际检验医学杂志》
CAS
2015年第6期731-733,共3页
International Journal of Laboratory Medicine
基金
兰州市科技发展计划项目(2012-1-12)
