摘要
目的研究Mi R-146a对脂多糖(LPS)导致的大鼠隐窝上皮细胞(IEC-6)的损伤效应。方法将复苏后的IEC-6细胞分为三组:正常组(无LPS,N组),脂多糖组(LPS,L组)和Mi R-146a治疗组(LPS+Mi R-146a,H组)。在H组,Mi R-146a在LPS刺激30 min后立即加入细胞培养基中。LPS刺激2 h后收集细胞用于氧自由基(ROS)测定,LPS刺激12 h后,离心细胞用于超氧化物歧化酶(SOD)和丙二醛(MDA)的测定;LPS刺激24 h后,收集细胞用于细胞凋亡和细胞活力检测,并用Hoechst33342进行细胞凋亡的检测。结果 Mi R-146a抑制了LPS导致的ROS的过量产生,减轻了氧化应激的损伤,进而减轻了LPS诱导的IEC-6细胞的凋亡和活力的下降。结论 Mi R-146a可以减轻LPS造成的IEC-6细胞的损伤。
Objective To investigate the protective effect of Mi R-146 a on lipopolysaccharide（LPS）-induced rat intestinal epithelial cells（IEC-6） damage. Methods IEC-6 cells were divided into three groups： normal group（No LPS, group N）, LPS group（LPS, Group L） and Mi R-146 a treatment group（LPS＋Mi R-146 a treatment, Group H）. In group H, Mi R-146a（10 μg/ml） was administered just after LPS（2 mg/L） treatment. Cells were collected for ROS determination at 2 hours after LPS stimulation. The remained cells were used for malonaldehyde（MDA） and superoxide dismutase（SOD） detection at 12 hours post-injury. Twenty-four hours post-injury, IEC-6 cells were used for cell apoptosis and viability.Furthermore, the cells were also detected with Hoechst 33342 to visualize the nuclei. Results Mi R-146 a inhibited the over-produced of ROS caused by LPS. In addition, Mi R-146 a lessened the LPS-induced oxidative stress damage in IEC-6 cells. Finally, Mi R-146 a reduced the LPS-induced cell apoptosis and the decrease of cell viability. Conclusion Mi R-146 a is an effective method for LPS-induced IEC-6 cell damage.
出处
《中华临床医师杂志（电子版）》
CAS
2015年第6期66-68,共3页
Chinese Journal of Clinicians(Electronic Edition)
