摘要
采用薄膜分散法制备格列齐特脂质体,以粒径和包封率为考核指标,通过单因素实验和正交实验优化制备条件,测定最优条件制备格列齐特脂质体的平均粒径和包封率。确定最优制备条件为:药脂比1∶10(g∶g)、超声时间10min、成膜温度60℃、缓冲液pH值6。所制备脂质体的平均粒径为(108.3±12.4)nm、包封率为(72.19±3.6)%、平均Zeta电位为(-40.8±2.3)mV,且在4℃下保存稳定性好。电镜照片显示,所制备脂质体圆整度好、粒径均一、无粘连。表明采用薄膜分散法制备格列齐特脂质体工艺稳定,质量可控。
Gliclazide liposomes were prepared by thin-film dispersion method.Using mean diameter and entrapment efficiency as evaluation index,the preparation conditions were optimized by single factor experiment and orthogonal experiment,and mean diameter and entrapment efficiency were measured.The optimal conditions were as follows：drug-lipid ratio of 1∶10（g∶g）,ultrasonic time of 10 min,film-forming temperature of 60℃,the buffer solution pH value of 6.The mean diameter,entrapment efficiency and average Zeta potential of gliclazide liposomes were（108.3±12.4）nm,（72.19±3.6）% and（-40.8±2.3）mV,respectively.The liposomes showed good stability under preservation at 4 ℃.TEM Image showed that the liposomes exhibited good spherical degree,uniform particle size and no adhesion.The results showed that the process of gliclazide liposomes prepared by thin-film dispersion method was stable and the quality could be controlled well.
出处
《化学与生物工程》
CAS
2015年第3期40-44,共5页
Chemistry & Bioengineering
关键词
格列齐特
脂质体
薄膜分散法
包封率
gliclazide
liposome
thin-film dispersion method
entrapment efficiency
