摘要
[ Objective] To detect the praziquantel content in praziquantel injection by HPLC. [ Methods ] Chromatographic conditions were as follows： chromato- graphic colmnn was Diamonsil Cls column （ 150 mm x 4.6 mm, 5 μm） ; column temperature was 25 ℃ ; mobile phase was acetonitrile-water （60：40） ; flow rate was 1.0 mL/min ; UV detection wavelength was 210 nm; and injection volume was 20 μL. [ Results ] Praziquantel showed good linear relationship within the con- centration of 6.037 - 90.55 g/L （ r = 0. 999 4） ; the average recovery rate was 99.24%, and RSD was 0. 769%. [Condusions ] This method was simple, rap- id, sensitive and repeatable, and could be used for the quality control of praziquantel injection.
[ Objective] To detect the praziquantel content in praziquantel injection by HPLC. [ Methods ] Chromatographic conditions were as follows： chromato- graphic colmnn was Diamonsil Cls column （ 150 mm x 4.6 mm, 5 μm） ; column temperature was 25 ℃ ; mobile phase was acetonitrile-water （60：40） ; flow rate was 1.0 mL/min ; UV detection wavelength was 210 nm; and injection volume was 20 μL. [ Results ] Praziquantel showed good linear relationship within the con- centration of 6.037 - 90.55 g/L （ r = 0. 999 4） ; the average recovery rate was 99.24%, and RSD was 0. 769%. [Condusions ] This method was simple, rap- id, sensitive and repeatable, and could be used for the quality control of praziquantel injection.
基金
Supported by the Planning Program for Science and Technology Development of Taizhou City(TG1126)
the Research Project of Jiangsu Agri-animal Husbandry Vocational College(NSFFH1307)
