摘要
为了解上海地区规模化猪场中粪肠球菌的耐药性和基因型,2013年3月-2014年5月从上海地区10个规模化养猪场收集到分离鉴定的粪肠球菌133株,采用微量肉汤稀释法对12种抗菌药物进行药敏试验,采用PCR技术分别检测分离株中β-内酰胺类抗生素耐药相关基因（TEM）、氨基糖苷类抗生素耐药相关基因[aac（6’）/aph（2＂）和aph（3’）-Ⅲ和ant（6）-Ⅰ]、四环素耐药相关基因（tetM）、红霉素耐药相关基因（ermB和mefA）和万古霉素耐药相关基因（vanA和vanB）。结果显示,在133株粪肠球菌中,耐药基因ermB、tetM、TEM、ant（6）-Ⅰ、aac（6’）/aph2＂、aph（3’）-Ⅲ、vanA的检出率分别为：95.5%（127/133）、91.0%（121/133）、63.2%（84/133）、45.9%（61/133）、42.1%（56/133）、24.1%（32/133）、3.0%（4/133）;未检出mefA和vanB基因的菌株。分离菌株对12种抗菌药物的耐药性较为严重,且以6-9耐为主要耐药株,占81.2%。试验表明,上海地区猪源粪肠球菌多重耐药现象严重,携带抗菌药物相关耐药基因是导致分离株耐药的主要原因。
This study was conducted to investigate the antibiotic susceptibility and resistance genotypes in Enterococcus faecalis strains isolated from ten Large-scale pig farms in Shanghai. 133 strains of E. faecalis were collected between March 2013 and May 2014. The susceptibility testing was performed by broth microdilution, and the genotypes of β-lactam antibiotics resistance-associated gene（TEM）, aminoglycoside genes[aac（6’）/aph（2”）, aph（3’）-Ⅲ and ant（6）-Ⅰ], tetracycline gene（tetM）, erythromycin genes（ermB and mefA） and vancomycin genes（ vanA and vanB ） were analyzed by PCR. The positive rate of the resistance genes ermB, tetM, TEM, ant（6）-Ⅰ, aac（6’）/aph2”, aph（3’）-Ⅲ, vanA in the 133 strains of E. faecalis tested were 95.5%（127/133）, 91.0%（121/133）, 63.2%（84/133）, 45.9%（61/133）, 42.1%（56/133）, 24.1%（32/133）, and 3.0%（4/133）, respectively. No mefA and vanB gene strains were detected. 12 kinds of antibacterial drug resistance were relatively serious and 6-9 resistance as the main drug resistant strains were detected,accounting for 81.2%. This study shows that multidrug resistance of E. faecalis was a serious issue, and resistance genes harbored were the very important reasons for resistance to antibiotics in E. faecalis.
出处
《中国兽药杂志》
北大核心
2015年第2期13-17,共5页
Chinese Journal of Veterinary Drug
关键词
粪肠球菌
耐药性
基因型
抗菌药物
Enterococcus faecalis
antimicrobial resistance
genotype
antimicrobial agents
