摘要
通过盆栽实验,研究硝化抑制剂硫脲对大豆苗期的生物量、叶片金属离子含量、叶绿素含量、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和丙二醛(MDA)含量的影响.结果表明,大豆的幼苗对土壤中硫脲的浓度较为敏感,低浓度硫脲(1.0 mmol kg-1)就能轻微抑制大豆的生长,当硫脲浓度达到5.0 mmol kg-1时,大豆幼苗的生长受到明显的抑制.不同硫脲浓度种植条件下,大豆幼苗体内的铜离子和锌离子含量变化不大,而锰离子和铁离子含量却表现出明显差异.锰离子含量随硫脲浓度的升高而升高,在高浓度硫脲(5.0 mmol kg-1)条件下由硫脲空白实验的18.58 mg kg-1上升到45.45 mg kg-1,增加了一倍以上.而铁离子含量随硫脲浓度的升高而降低,在高浓度硫脲(5.0mmol kg-1)条件下由硫脲空白实验的20.77 mg kg-1下降到17.63 mg kg-1.高浓度的硫脲影响了大豆苗对铁的吸收,从而影响了叶绿素的合成,造成叶片叶绿素含量降低,由低浓度硫脲条件下最高的4.12 mg kg-1下降到2.15 mg kg-1.研究结果还表明,低浓度硫脲能使大豆叶片中SOD、POD和CAT的活性提高,但当硫脲浓度升高时,其活性降低.同时,当硫脲浓度达到5.0 mmol kg-1时,叶片MDA含量明显上升.因此,大豆叶片中SOD、POD、CAT等酶的活性降低和MDA含量的上升表明大豆幼苗在高浓度硫脲种植条件下受到明显的环境胁迫和毒害作用.
For a precise evaluation of environmental and eco-toxicology effect of nitrification inhibitor on agroecosystem,the phytotoxicity of nitrification inhibitor should be fully understand.This paper aimed to study the phytotoxicity of thiourea and urea application on soybean seedlings.Effects of thiourea on the biomass,content of metal ions and chlorophyll level were studied in the leaves of soybean,and the effects of thiourea on anti-oxidative system（SOD,POD,and CAT） and content of MDA in the leaves of soybean were also investigated.The results showed that thiourea had great impact on the growth of soybean.Low concentration（1.0 mmol kg^-1） of thiourea could slightly inhibit the growth of seedlings,and increase the activities of SOD,POD,CAT.Thiourea treatment of different concentrations had no effect on the content of Cu and Zn ion in soybean seedlings,but could obviously change the content of Mn and Fe ions in soybean seedlings.The content of Mn ion increased with the concentration of thiourea,from 18.58 mg kg^-1 in the control to 45.45 mg kg^-1 with 5 mmol kg^-1 thiourea treatment.On the other hand,the content of Fe ion decreased with the concentration of thiourea,from 20.77 mg kg^-1 in the control to 17.63 with 5 mmol kg^-1 thiourea treatment.Heave inhibition effects and phytotoxic symptom were found in thriourea treatment of 5.0mmol kg^-1.The growth of seedlings was obviously inhibited;the content of chlorophyll in leaves of soybean decreased to 2.11 mg kg^-1 compared to 3.56 mg kg^-1in that of the control.Simultaneously,the activities of SOD,POD,CAT and the content of MDA increased dramatically when thiourea reached 5.0 mmol kg^-1.The heave inhibition effects and phytotoxic symptom in the soybean seedling indicated that thiourea could induce environmental stress in the seedlings.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2015年第1期175-180,共6页
Chinese Journal of Applied and Environmental Biology
基金
海南省高等学校科研项目(Hjkj2013-46)
国家自然科学基金项目(30770389)
三亚学院人才引进项目资助~~
关键词
硫脲
大豆
生物量
叶绿素
酶活性
thiourea
soybean
biomass
chlorophyll
enzymes
