摘要
目的观察Th22细胞在小鼠外周神经异种移植排斥反应中的动态变化。方法40只C57小鼠按随机数字表法分为对照组和实验组，每组20只。实验组小鼠行异种异体坐骨神经移植（供体为预先切除的SD大鼠坐骨神经），对照组小鼠行自体坐骨神经移植。利用Catwalk系统检测2组小鼠术后坐骨神经功能的变化情况：应用免疫组化染色检测移植神经周围组织中IL-22阳性细胞不同时间点的浸润情况。流式细胞术检测脾细胞Th22细胞比例的变化：ELISA法检测血清IL-22的水平。结果（1）Catwalk系统检测结果显示，实验组小鼠主要指标均明显差于对照组小鼠，差异均有统计学意义（P〈0．05）。（2）免疫组化染色结果显示，实验组小鼠移植神经周围组织中浸润的IL-22阳性细胞数量呈现出逐渐增多的趋势，并且在术后第5、15、25天显著高于对照组小鼠，差异均有统计学意义（P〈0．05）。（3）流式细胞仪检测结果显示，实验组小鼠脾内T1122细胞百分比在术后第5、15、25天分别为（7．76±0．81）％、（9．6±0．73）％、（8．08±0．66）％，明显高于对照组的（5．10±0．28）％、（5．13±0．35）％、（4．92±0．34）％，差异均有统计学意义（P〈0．05）。（4）ELISA结果显示，实验组小鼠术后第5、15、25天血清IL-22水平分别为（8．99±0．94）pg／mL、（11．0±1．08）pg／mL、（8．65±1．04）pg／mL，明显高于对照组的（5．16±0．13）pg／mL、（5．22±0．22）pg／mL、（4．96±0．29）pg／mL），差异均有统计学意义（P〈0．05）。结论Th22细胞可能参与小鼠外周神经异种移植排斥反应发生过程。
Objective To evaluate the dynamic changes of Th22 cells in rejection of the peripheral nerve xenotransplantation in mice. Methods Forty healthy male C57 BL/6 mice were randomly divided into isograft group and xenograft group （n=20）. Catwalk automated gait analysis system was used to evaluate the motor function changes after isograft/xenograft treatment; immunohistochemical staining was used to observe to the interleukin （IL）-22 positive cell invasion degrees around the grafts at different time points. The percentages of Th22 cells in spleen were detected by flow cytometry; serum IL-22 levels were assessed by ELISA. Results Catwalk-assisted gait analysis showed that xenograft group had significantly poorer motor and neurological functions as compared with isograft group （P〈0.05）. The degrees of IL-22^± cell invasion around the grafts in xenograft group significantly increased 5, 15, and 25 d after xenotransplantation as compared with those in the isograft group （P〈0.05）. As compared with those in the isograi± group （[5.10±0.28] %, [5.13±0.35] % and [4.92±0.34] %）, markedly increased percentages of Th22 cells （[7.76±0.81] %, [9.6±0.73] % and [8.08±0.66] %） were noted in the xenograft group 5, 15, and 25 d aider xenotransplantation （P〈0.05）. ELISA results revealed that the serum IL-22 levels during rejection in the xenograft recipients （[8.99±0.94] pg/mL, [11.0±1.08] pg/mL and [8.65 ±1.04] pg/mL） were statistically higher than those in the control group （[5.16±0.13] pg/mL, [5.22±0.22] pg/mL and [4.96±0.29] pg/mL） 5, 15, and 25 d after xenotransplantation （P〈0.05）. Conelnsion Th22 cells contribute to the rejection process of peripheral nerve xenotransplant.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2015年第1期9-13,共5页
Chinese Journal of Neuromedicine
基金
国家自然科学基金（30901546）
