摘要
以灰木莲(Manglietia glauca)的新鲜花粉为材料,研究不同浓度蔗糖、硼酸和氯化钙组合的培养基对灰木莲花粉离体萌发的影响,并用TTC染色法、I-IK染色法和无机酸法对灰木莲的花粉生活力进行了测定。结果表明:在p H值6.0时,以150 g/L蔗糖+50 mg/L硼酸+100 mg/L氯化钙+7 g/L琼脂的培养基配方最适合灰木莲花粉的培养,离体萌发率达到62.2%,花粉管生长良好;无机酸法不适宜灰木莲花粉生活力的检测;TTC染色法和I-IK染色法的测定结果与离体萌发法的结果较接近,分别为60.6%和64.1%,但I-IK染色法在观察判断上较为困难。因此,TTC染色法是快速检测灰木莲花粉生活力的最佳方法。
The fresh pollen of Manglietia glauca was used as material to study the effect of mediums with different concentrations of sucrose, boric acid and calcium chloride on in vitro pollen germination . And the pollen viabilities of M. glauca were tested by using 2 , 3 , 5-triphenyltetrazolium chloride staining （ TTC） , iodine-potassium iodide staining （ I-IK） and mineral acid test. The results showed that when the pH was 6. 0, the optimum medium consisting of 150 g/L sucrose, 50 mg/L boric acid, 100 mg/L calci-um chloride and 7 g/L agar was suitable for M. glauca pollen cultured. The in vitro germination rate was 62. 2% and the pollen tube grew well. The mineral acid test was unsuitable for detecting the pollen via-bility. The results tested by TTC and I-IK methods were similar to that of in vitro germination method with pollen viabilities 60. 6% and 64. 1% respectively. However, it was difficult to distinguish the stained pollen grains using the I-IK method. The TTC method was the optimum test method for testing pollen via-bility of M. glauca rapidly.
出处
《广西林业科学》
2014年第4期405-408,共4页
Guangxi Forestry Science
基金
广西科学研究与技术开发计划项目(桂科攻11194008)
关键词
灰木莲
花粉
离体萌发
生活力
花粉管
Manglietia glauca
pollen
in vitro germination
viability
pollen tube
