摘要
目的探讨乳腺癌BAG-1基因表达与表皮生长因子受体(EGFR)表达的相关性。方法培养乳腺癌细胞株,采用实时聚合酶链反应技术检测EGFR(+)和EGFR(-)细胞株中BAG-1 m RNA的表达情况,West blot法检测其蛋白水平。分析BAG-1基因表达与EGFR表达的相关性。结果 MDA-MB-231、T47D细胞株EGFR m RNA和EGFR蛋白阳性表达,MCF-7、SLBP3细胞株EGFR m RNA和EGFR蛋白阴性表达。T47D细胞株BAG-1 m RNA的表达水平最高[(29.6±0.4)×10-4],其次为MDA-MB-231细胞株[(6.9±0.3)×10-4],两者的表达水平均显著高于MCF-7细胞株[(4.3±0.5)×10-4]、SKBR3细胞株[(0.3±0.1)×10-4],差异的有高度统计学意义(P<0.01)。结论BAG-1表达与EGFR存在相关性,可作为乳腺癌靶向治疗的潜在靶点。
Objective To discuss the correlation of BAG-1 gene expression and EGFR expression in breast cancer.Methods Breast cancer cell lines were cultured, BAG-1 m RNA expression was detected by PCR in EGFR（＋） and EGFR（-） cell lines. BAG-1 protein expression was detected by West blot. Correlation of BAG-1 gene expression and EGFR expression in breast cancer was analyzed. Results EGFR m RNA and EGFR protein were positive expression in EGFR MDA-MB-231 and T47 D cell lines, and negative expression in MCF-7 and SLBP3 cell lines. BAG-1 m RNA expression was highest in T47 D cell line [（29.6±0.4）×10-4], followed by MDA-MB-231 cell line [（6.9±0.3）×10-4]. Both expression levels were significantly higher than that of MCF-7 cell line [（4.3±0.5）×10-4] and SKBR3 cell line [（0.3±0.1）×10-4],the differences were statistically significant（P〈0.01）. Conclusion Expression of BAG-1 shows correlation with EGFR,which can be used as a potential targets for targeted therapy of breast cancer.
出处
《中国医药导报》
CAS
2014年第36期25-29,共5页
China Medical Herald
基金
浙江省医药卫生平台骨干人才计划项目(编号2011RCA011)
