摘要
目的建立虎柏烧伤酊中大黄素、虎杖苷和盐酸小檗碱定量测定方法。方法采用Eclipse XDB-C18（150 mm×4.6mm,5μm）色谱柱,流动相为乙腈-0.1%磷酸水溶液,梯度洗脱,体积流量1.0 mL/min,检测波长265 nm（盐酸小檗碱）、306 nm（大黄素和虎杖苷）,柱温30℃。结果大黄素在6.3-200.0 ng、虎杖苷在9.6-308.0 ng、盐酸小檗碱在5.0-160.0ng有良好的线性关系,样品中大黄素、虎杖苷和盐酸小檗碱的平均加样回收率分别为99.91%、99.51%和99.16%,RSD分别为1.12%、0.84%和1.17%。结论本法结果准确,精密度及重复性较好,可作为虎柏烧伤酊的质量控制方法。
Objective To establish a method for determining the contents of emodin, polydatin, and berberine hydrochloride in Hubai Burrn Tincture. Methods The samples were separated on an Eclipse XDB-C18（150 mm × 4.6 mm, 5 μm） column by a gradient elution using acetonitrile and 0.1% phosphoric acid aqueous solution as mobile phase at a flow rate of 1 mL/min. The eluate was detected by double wavelengths： 265 nm for berberine hydrochloride and 306 nm for emodin and polydatin. Column teperature was set at 30 ℃. Results The linear ranges of emodin, polydatin, and berberine hydrochloride were 6.3—200.0, 9.6—308.0, and 5.0—160.0 ng. The average recovery rates of emodin, polydatin, and berberine hydrochloride were 99.91%（RSD = 1.12%）, 99.51 %（RSD = 0.84%）, and 99.16%（RSD = 1.17%）. Conclusion This method is accurate, specific, and reliable for the quality control of Hubai Burrn Tincture.
出处
《中草药》
CAS
CSCD
北大核心
2014年第20期2932-2934,共3页
Chinese Traditional and Herbal Drugs
