摘要
目的比较在牙龈卟啉单胞菌脂多糖(Pg-LPS)作用下,健康和高脂血症兔肺泡巨噬细胞(AM)膜表面清道夫受体-A(SR-A)的基因表达变化以及白细胞介素-10(IL-10)对其的调控作用。方法将健康新西兰兔12只随机分为2组,分别给予普通和高脂饲料喂养,6周后建立高脂血症模型,采用肺泡灌洗法分别获得健康和高脂组AM,将健康和高脂组AM随机分为对照组、加1μg/mL Pg-LPS组、加1μg/mL Pg-LPS+0.1μg/mL IL-10组,24h后裂解细胞,荧光定量PCR法检测SR-A的基因表达。结果健康及高脂血症Pg-LPS组SR-A的表达均较对照组明显升高(P<0.05),且高脂Pg-LPS组较健康Pg-LPS组升高更加显著(P<0.05);在加入IL-10后,健康组SR-A的表达高于单纯Pg-LPS组(P<0.05),高脂组SR-A的表达则低于单纯Pg-LPS组(P<0.05)。结论牙周致病菌Pg-LPS可增加高脂血症兔AM膜SR-A的表达,从而加大动脉粥样硬化的发病风险,并且可能受到抗炎因子IL-10的负调控。
Objective To compare the expression of alveolar macrophages membrane receptor SR-A mRNA ,stimulated by Porphyromonas gingivalis lipopolysaccharide （Pg-LPS） in healthy and hyper-lipidemic rabbit ,and detect the effect of interleukin10（IL-10） . Methods 12 healthy New Zealand rabbits were randomly divided into two groups ,and respectively gave them normal and high fat diets . After 6 weeks ,hyperlipidemia model was established . Alveolar macrophages （AM ） of both healthy and hyper-lipidemia groups were isolated by alveolar lavage and then further divided into three groups ：control , 1 μg/mL Pg-LPS and 1 μg/mL Pg-LPS＋0 .1 μg/mL IL-10 . After the treatment with Pg-LPS and IL-10 for 24 hours ,the mRNA expression levels of SR-A in all samples were detected by real-time fluorescence quantitative polymerase chain reaction （PCR） . Results Results of real-time PCR showed that the level of SR-A on both healthy and hyperlipidemic rabbit AM were significantly increased （P〈0 .05） by Pg-LPS when compared with control group . Moreover ,the increasing of SR-A level in hyperlipidemic Pg-LPS group was more prominent than in healthy Pg-LPS group（P〈0 .05） . Furthermore ,when compared with that in Pg-LPS group ,IL-10 induced higher SR-A expression in healthy group and lower SR-A expression in hyperlipidemic group （P〈0 .05） . Conclusion Periodontal pathogen Pg-LPS might increase the risk of atherosclerosis by stimulating the expression of alveolar macrophages membrane receptor SR-A in hyper-lipidemia ,and this stimulation could be negatively regulated by IL-10 .
出处
《福建医科大学学报》
2014年第4期213-216,共4页
Journal of Fujian Medical University
基金
国家自然科学基金(81170973
30973326)
福建省医学创新课题(2011-CX-26)
