摘要
设计了一种新颖实用的制备L-别苏氨酸和D-别苏氨酸的方法。以α-C消旋的N-乙酰化苏氨酸为原料,用L-(或D-)氨基酰化酶拆分相应的底物得到了高光学纯度的L-别苏氨酸(或D-别苏氨酸)。为了获得较高收率,考查p H,温度,Co2+浓度对拆分反应的影响。确定最优条件为:p H 7.5,温度37℃,Co2+浓度0.1 g·L-1。反应产率达到93.8%(L-allo-Thr)、89.8%(D-allo-Thr),de>99%。该方法具有立体选择性高,操作简单,反应条件温和的优点。
A novel and practical approach for preparation of L-allothreonine and D-allothreonine was designed. The optical purity L-allo-Thr（ or D-allo-Thr） compound was obtained by using the L-（ or D-） aminoacylase to resolute the α-C racemic threonine of N-acetylation. The resolution conditions were optimized by assessing pH,temperature and the amount of Co2＋ for obtaining the maxi-mum yield. The optimal condition was that pH was 7. 5,reaction temperature was 37℃,and concentration of Co2＋was 0. 1 g·L-1 . The yield was 93. 8%for L-allo-Thr,89. 8%for D-allo-Thr,and de〉99%. This method showed some advantages,such as high ste-reoselectivity,simple operation and mild conditions.
出处
《化学研究与应用》
CAS
CSCD
北大核心
2014年第12期1940-1943,共4页
Chemical Research and Application
基金
"973"计划项目(2012CB723501)资助
关键词
别苏氨酸
氨基酰化酶
拆分
消旋
allo-threonine
aminoacylase
resolution
racemization
