摘要
稻瘟病菌与禾谷镰刀菌均是危害禾本科作物的重要病菌,同时也是研究植物病原真菌的两种模式生物。为进一步探索这两种病菌的致病能力和侵染过程,对稻瘟病菌与禾谷镰刀菌在茭白上的致病能力进行了测定,并比较了二者在茭白不同部位的发病过程及特点。结果显示,稻瘟病菌可侵染茭白叶片与叶鞘,但无法侵染茭白的肉质茎(茭白笋);而禾谷镰刀菌不能侵染未创伤的茭白叶片和叶鞘,但可对茭白笋造成危害。对侵染部位的显微观察发现,稻瘟病菌在茭白叶片上能形成典型的附着胞与侵染菌丝。另外,茭白能有效地区分两种病菌的致病突变体。结果表明,稻瘟病菌与禾谷镰刀菌能够侵染茭白,造成危害;同时茭白也可以作为寄主研究这两种病菌的致病过程。
Magnaporthe oryzae and Fusarium graminearum are two important plant pathogens,which cause destructive diseases on gramineous crops,and are also two model organisms for the study on plant fungal pathogens. To reveal the infection ability and invasion process of the two pathogens on Zizania caduciflora,their pathogenicity were tested,and the infection processes on different parts of Z. caduciflora were compared. Our data showed that M.oryzae could infect the leaves and leaf sheaths rather than the fleshy stems of Z. caduciflora. Contrarily,F. graminearum generated symptoms on fleshy stems,but failed to infect unwounded leaves or leaf sheaths of Z. caduciflora. Fluorescent microscopy of the lesions showed that M. oryzae performed normal invasion process on leave surface of Z. caduciflora,producing typical appressoria and infection hyphae. In addition,the pathogenicity-altered mutants of the two fungi can be easily differentiated on Z. caduciflora. These findings indicate that M. oryzae and F. graminearum can infect Z. caduciflora and make potential damage; meanwhile,Z. caduciflora can be an alternative host used in the research on the pathogenesis of the two pathogens.
出处
《浙江农业学报》
CSCD
北大核心
2014年第6期1546-1551,共6页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(30900933
31170136
30970082)
973前期资助项目(2012CB722504)
关键词
稻瘟病菌
禾谷镰刀菌
茭白
致病性
侵染过程
Magnaporthe oryzae
Fusarium graminearum
Zizania caduciflora
pathogenicity
Infection process
