摘要
目的 探明汉坦病毒汉滩型与希望山型之间能否发生基因重排 ,以及发生重排的频率和特点 .方法 用汉坦病毒汉滩型 76 - 118株与希望山型 PHV株混合感染 Vero E6细胞 ,空斑形成实验挑取子代病毒克隆株 ,传代培养后用 RT-PCR方法鉴定子代病毒基因型 .结果 子代病毒 14 /36来源于 PHV株 ;12 /36来源于 76 - 118株 ;5 /36汉滩型与希望山型分型引物扩增均为阳性 ;1株子代病毒 L,S片断均来源于76 - 118株 ,而 M片段两种引物扩增均为阳性 ;2株子代病毒 L片断来源于 76 - 118株 ,S片断来源于 PHV株 ,M片段两种引物扩增均为阳性 . 1株子代病毒 L ,S片断均来源于 76 - 118株 ,而 M片段来源于 PHV株 .
AIM To determine the frequency and characteristics of gene segments reassortment between Hantaan virus and Prospect Hill virus. METHODS Mixed infections were initiated in tissue culture by using Hantaan virus strains (76 118) and Prospect Hill Virus Strains (PHV). Potential reassortant virus plaques were screened by multiplex RT PCR, using primers specific for individual genome segments of each strain. RESULTS Most of the progeny virus plaques (28 of 36) had the parental genotype of 76 118 strain or PHV strain. 5 of 36 plaques had mixed genotypes that yielded RT PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 4 of 36 progeny plaques tested. In addition, approximately 8.33% of the progeny virus plaques appeared to contain M segments originating from both parental virus strains, i.e., they were diploid. CONCLUSION Genetic reassortment can occur between Hantaan virus strains and Prospect Hill virus strains.
出处
《第四军医大学学报》
北大核心
2002年第14期1290-1292,共3页
Journal of the Fourth Military Medical University
基金
国家自然科学基金资助项目 (3 9670 65 0 )
