摘要
目的观察基底细胞样乳腺癌(BLBC)组织中凋亡蛋白酶活化因子1(Apaf-1)、细胞凋亡蛋白酶9(Caspase-9)的表达变化,并探讨其意义。方法采用免疫组化法检测43例BLBC(A组)、57例non-BLBC(B组)和60例癌旁正常乳腺组织(C组)Apaf-1、Caspase-9的表达,分析两指标间及与BLBC临床病理参数的关系。结果A、B、C组Apaf-1阳性率分别为23.3%(10/43)、49.1%(28/57)、75%(45/60),两两比较,P均<0.01;Caspase-9阳性率分别为27.9%(12/43)、54.4%(31/57)、85%(51/60),两两比较,P均<0.01。Apaf-1表达与BLBC肿瘤直径、淋巴结转移、临床分期有关(P均<0.05),Caspase-9表达与BLBC淋巴结转移、临床分期有关(P均<0.05)。BLBC组织Apaf-1与Caspase-9阳性表达呈正相关(r=0.639,P<0.01)。结论 BLBC组织Apaf-1、Caspase-9表达降低,两者异常表达可能与BLBC的发生、发展密切相关。
Objective To observe the changes of Apaf-1, Caspase-9 expression in the basal-like breast cancer （ BLBC） tissues and explore its clinical significance.Methods The expressions of Apaf-1 and Caspase-9 were detected in 43 cases of BLBC（ group A） , 57 cases of non-BLBC（ group B） and 60 cases of normal breast tissue（ group C） by immuno-histochemistry.The relationship between Apaf-1, Caspase-9 expression and clinicopathological features were analyzed.The correlation between Apaf-1 and Caspase-9 expression was analyzed.Results The positive expression rate of Apaf-1 in group A, B, C was 23.3%（10/43）, 49.1%（28/57）, 75%（45/60） respectively.The difference between each group was significant（all P〈0.01）.The positive expression rate of Caspase-9 in group A, B, C was 27.9%（12/43）, 54.4%（31/57）, 85%（51/60） respectively.The difference between each group was all significant（all P〈0.01）.The expres-sion of Apaf-1 was significantly correlated with the size of tumor, lymph nodes metastasis and the tumor clinical stage（ all P〈0.05） .The expression of Caspase-9 was significantly correlated with lymph nodes metastasis and the tumor clinical stage （all P〈0.05）.There correlation between the expression of Apaf-1 and Caspase-9 was significant（r=0.636, P〈0.01）. Conclusions Apaf-1 and Caspase-9 are lower expressed in BLBC.The abnormal expression of Apaf-1 and Caspase-9 may be closely correlated with the carcinogenesis and development of BLBC.
出处
《山东医药》
CAS
2014年第39期12-14,共3页
Shandong Medical Journal
基金
河北省卫生厅医学科学研究课题(20130333)
