摘要
目的探讨艾司洛尔对脓毒症大鼠心肌细胞凋亡和心功能的影响。方法将96只SD大鼠分为假手术组、脓毒症组(盲肠结扎穿孔法建模)、治疗组(建模基础上艾司洛尔静脉给药),每组各32只,于术后3、6、12、24h(每个时间点8只)开腹留取标本检测心肌肌钙蛋白I(cTnI)、caspase-3活性、心肌细胞凋亡指数,光镜下观察心肌组织。第24小时用超声仪测定心功能。结果治疗组和脓毒症组各时间点cTnI、caspase-3活性和心肌细胞凋亡指数均较假手术组升高(P<0.05)。治疗组在应用艾司洛尔后cTnI和心肌细胞凋亡指数各时间点均有不同程度下降,caspase-3活性在12h后明显下降(P<0.05)。脓毒症组第24小时左室射血分数(LVEF)较治疗组下降更为显著(P<0.05)。结论艾司洛尔具有抑制大鼠脓毒症心肌细胞凋亡和减轻心脏收缩功能损害的作用,其作用可能与抑制caspase-3活性有关。
Objective To investigate the effect of esmolol on myocardial apoptosis and cardiac function in rats with sepsis .Meth‐ods Ninety six SD rats were randomly divided into sham group （n= 32 ,for sham operation） ,sepsis group （n= 32 ,sepsis model） and treatment group （n= 32 ,intravenous infusion with esmolol after sepsis modeling） .At 3 h ,6 h ,12 h ,24 h after operation ,sam‐ples were conserved to measure serum cardiac troponin I （cTnI） ,caspase‐3 ,cardiomyocytes apoptosis index ,and myocardial patho‐morphology was observed by optical microscope .At 24 h after operation ,echocardiography was performed .Results The serum cT‐nI ,caspase‐3 ,cardiomyocytes apoptosis index were increased significantly in sepsis group and treatment group compared with sham group （all P〈 0 .05） .After using esmolol ,cTnI and cardiomyocytes apoptosis index were relatively decreased in treatment group compared with sepsis group at the four time‐points（all P 〈 0 .05） .Caspase‐3 of treatment group decreased at 12 h after operation compared with sepsis group （P〈 0 .05） .Treatment group had a higher LVEF than sepsis group at 24 h（P〈 0 .05） .Conclusion Es‐molol could inhibit myocardial apoptosis and mitigate the damage of cardiac function in rats with sepsis .The decrease in caspase‐3 may be the mechanism of anti‐apoptosis .
出处
《重庆医学》
CAS
CSCD
北大核心
2014年第31期4181-4184,共4页
Chongqing medicine
