摘要
目的 探讨血白细胞介素-18 （ILq8）和白细胞介素-18结合蛋白（IL-18BP）水平在慢性乙型肝炎患者中的变化及临床意义.方法 分别应用酶联免疫吸附法和速率法检测100例慢性乙型肝炎患者血清IL-18、IL-18BP和丙氨酸氨基转移酶（ALT）水平,应用荧光定量逆转录聚合酶链反应（FQ-RT-PCR）检测乙型肝炎患者血清中乙型肝炎病毒（HBV） DNA载量,并以100例正常健康体检者作对照.结果 与正常对照组相比,慢性乙型肝炎患者组血清IL-18、IL-18 BP、ALT和HBV DNA载量水平明显增高（P＜0.05）;血清IL-18和IL-18BP、ALT和HBVDNA载量水平随着临床表现逐步加重逐步升高;血清IL-18水平与血清IL-18 BP、ALT和HBV DNA载量水平水平呈正相关（r=0.808,r=0.672,r =0.631,P均＜0.01）,IL-18BP水平与ALT、HBV DNA载量水平水平亦呈明显正相关（r=0.717,r=0.683,P均＜0.01）.结论 IL-18和IL-18BP共同参与慢性乙型肝炎免疫致病过程,且与肝脏炎症活动性、肝损害程度及预后密切相关.
Objective To investigate the changes and clinical significance of serum IL-18 and IL-18BP in patients with chronic HBV disease.Methods Serum IL-18,IL-18BP and ALT levels were measured by ELISA and velocity respectively in 100 patients with chronic HBV and 100 healthy cases control group,and HBV DNA levels were measured by FQ-RT-PCR.Results The serum levels of IL-l8,IL-18BP,ALT and HBV DNA were significant increased in chronic HBV patients,compared with normal control group（P ＜0.05）.Serum levels of IL-18,IL-18BP,ALT and HBV DNA increased when the clinical symptom gradually getting.The IL-18 level was positively correlated with serum levels of IL-18BP,ALT and HBV DNA（r =0.808,r =0.672,r =0.631,P all ＜0.01）,and the serum level of IL-18BP was also positively correlated with ALT and HBV DNA （r =0.717,r =0.683,P all ＜ 0.01）.Conclusions IL-18 and IL-18BP may participate in the pathogenesis of chronic HBV disease,and show correlation with the severity of histological inflammation and prognosis.
出处
《中国实用医刊》
2014年第23期17-19,共3页
Chinese Journal of Practical Medicine
