摘要
以杂交鹅掌楸体细胞胚为材料,研究体胚不同发育阶段、大量元素和维生素C、基因型、激素组合以及移栽基质对体胚苗萌发和移栽存活的影响。结果表明:鱼雷型胚萌发效果最好;3/4 MS培养基较适合体胚苗的萌发;Vc有利用保持体胚苗维持正常形态。不同基因型体胚苗萌发差异较大,基因型1×5002萌发效果最好,成苗率为84.26%;而1×4088仅有13.24%。KT 0.1 mg/L+IBA 0.1 mg/L有利于促进体细胞胚的萌发。不同基因型体胚苗移栽存活率有差异,黄心土较混合泥炭土更适合体胚苗移栽。
The influences of development period of somatic embryos, major element and vitamin C, genotypes, different combinations with plant growth regulators and transplant medium on germination and transplanting survival for somatic embryos of Liriodendrom hybrids were studied. The results showed that the optimal developement phase of germination was torpedo-shaped embryos, and the base cultural medium of 3/4 MS and with the vitamin C used of keeping normal form from somatic embryos. The somatic embryo germination among different genotypes at the same cultural condition was different significantly, for example, the genotype 1×5002 was the highest, up to 82.46%, the genotype 1×4088 just was to 13.24%. The medium KT 0.1 mg/L ＋ IBA 0.1mg/L can help to promote the germination of somatic embryogenesis. There were differences of seedling transplanting survival rate between the yellow subsoil and mixed peat soil, and the former was more suitable for transplanting than the later.
出处
《江西林业科技》
2014年第5期1-3,26,共4页
Jiangxi Forestry Science and Technology
基金
江西省科技计划项目"杂交马褂木体培苗繁育技术研究"(NO:20111BDH80037)
国家林业局科技推广示范项目2012[50]号部分内容
关键词
杂交鹅掌楸
体细胞胚
萌发
Liriodendrom hybrids
somatic embryos
germination
